Abstract. Fatmawati U, Meryandini A, Nawangsih AA, Wahyudi AT. 2019. Screening and characterization of actinomycetes isolated from soybean rhizosphere for promoting plant growth. Biodiversitas 20: 2970-2977. Actinomycetes which colonized plant rhizosphere has a vital role in improving plant growth by producing Indole-3-Acetic Acid (IAA). The aim of this study was to evaluate the potency of actinomycetes isolated from soybean rhizosphere as promoter agents for plant growth in vitro. Fifty actinomycetes isolates were successfully isolated from soybean rhizosphere. Based on the colorimetric methods, 35 isolates can produce IAA in various concentration, in the range of 0.46-30.6 mg/L. Seed germination assay using Ragdoll methods revealed that 26 isolates significantly promoted germination parameters, including the hypocotyl and the radicular length, the number of the lateral roots, and dry weight of the plant. Also, 14 from 26 isolates showed phosphate solubilizing activity in different phosphate-solubilizing index ranging from 1.25-2.62. Eight isolates were able to grow in N-free medium, indicating that these isolates have the ability in fixing nitrogen. Out of 23 from 26 isolates were detected to produce siderophore. All the tested isolates show chitinase production except ASR 55. Based on the observed parameters, it showed that there are four potential isolates (ASR 46, ASR 58, ASR 75 and ASR 76) as promising plant-growth promoters, phosphate solubilizer, nitrogen fixer, siderophore and chitinase producer. Based on the result of 16S rRNA sequence analysis, four potential isolates were identified as Streptomyces spp. in different taxa of strains and species.
This research aimed to improve creative thinking ability and to reduce misconception on ecology in the students of X MIPA 3 of SMA Negeri 6 Surakarta in the academic of 2014/2015 through the application of e-module based on Problem-Based Learning. This research was a Classroom Action Research conducted in two cycles. Each cycle consisted of four stages: planning, acting, observing, and reflecting. The subject of research was the X MIPA 3 graders of SMA Negeri 6 Surakarta in the academic of 2014/2015 consisting of 9 boys and 21 girls. Techniques of collecting data used were essay test to measured creative thinking ability, open ended reasoning of two-tier diagnostic test to measure misconception, observation and interview as the proponent data. The data were analyzed using descriptive qualitative technique consists of three components: data reduction, data presentation, and taking the conclusion. Data validation of this research was triangulation method. Target of the research is an increase 20% on creative thinking aspects and decrease misconception 20% on ecology concepts at the end of the cycle. The result of research showed that there was an increase in each aspect of creative thinking ability: 24.68% of fluency, 34.17% of flexibility, 50% of originality, and 21.67% of elaboration. On the other side, the result of research also showed that there was a decrease in misconception in the important concepts of ecology including: 43.42% of population, 32.78% of community, 20.6% of food chain and web, and 47.92% of the ecology concept in science, environment, technology and community (salingtemas). The conclusion of this research described that the application of e-module based on Problem-Based Learning is able to improve the ability of creative thinking and reduce the students' misconception in ecology.
Salah satu domain penerapan patient-centered-cared yang dapat dilakukan adalah dengan mendengarkan pendapat pasien tentang asuhan keperawatan yang diharapkan serta memotivasi pasien untuk selalu terlibat aktif dalam proses perawatan. Metode pelibatan pasien: feedback pasien dan edukasi Speak-up merupakan cara yang dapat diterapkan di rumah sakit. Studi ini bertujuan menerapkan pengembangan metode pelibatan pasien: feedback pasien dan edukasi speak-up. Metode yang digunakan adalah pilot-study terhadap 57 perawat dengan menggunakan lembar observasi proses feedback pasien dan edukasi Speak-up yang terdiri dari 17 item. Tahapan dimulai dengan identifikasi masalah, analisis fishbone, implementasi dan evaluasi. Implementasi berupa pembuatan panduan, standar prosedur operasional, formulir, dan video role-play. Hasil studi menunjukan bahwa metode pelibatan pasien: feedback pasien dan edukasi speak-up dilakukan dengan capaian 69,42%. Keberhasilan proses pelibatan pasien dipengaruhi adanya keinginan organisasi dan staf untuk mendengarkan pasien, kesiapan untuk berubah, sumber daya yang mendukung, serta dukungan perawat, perawat manajer dan manajemen. Studi ini dapat menjadi dasar penerapan metode baru mengenai proses pelibatan pasien di rumah sakit. Kata kunci: feedback pasien, pelibatan pasien, speak-up
Actinomycetes are the one of the components of the rhizospheric microbial population and useful for producing secondary metabolites such as lytic enzymes, antibiotics, and antifungal. The aim of the study was to isolate the actinomycetes from maize rhizosphere collected from Kupang, East Nusa Tenggara. The screening was focused on the actinomycetes that showed the ability to produce antibacterial, antifungal, and extracellular enzymes such as amylase, cellulase, and protease. The actinomycetes were isolated using Humic-Acid Vitamin B (HV) agar media. The antagonistic assay was tested against Escherichia coli, Staphylococcus aureus, Sclerotium rolfsii and Fusarium oxysporum. Isolate JKP-8 was an isolate that showed the highest activity in inhibiting the growth of E. coli and S. aureus bacteria. Isolate JKP-5 showed the highest activity in inhibiting the growth of F.oxysporum. There were no actinomycetes isolates that showed an ability to inhibit the growth of S. rolfsii fungus based on dual culture assay. JKP-3 and JKP-4 isolates exhibited the highest ability to hydrolyze amylum, while JKP-5 and JKP-8 isolates exhibited the highest ability to hydrolyze CMC. The results of the amplification of 16S rRNA gene in selected potential isolates JKP 5 and JKP 8 indicated that both isolates belong to the genus Streptomyces.
<p>This research’s aim is to increase the problem solving skill of the students in X-A of SMA Negeri Sragen Billingual Boarding School Gemolong academic Year 2013/2014. The matter is about Enviroment Pollution through Problem Based Learning application by using local area potention. This research is a class action research which consists of three cycles. Each cycle contains of planning, implementing, observing and reflecting. The research subject is the students in X-A of SMA Negeri Sragen Billingual Boarding School Gemolong academic Year 2013/2014. Data of research were obtained by test and non test technique. Data validation were used triangulation of methods and triangulation of observers. The obtained data were analyzed using descriptive technique.The results of this research showed that according triangulation the average percentage for IDEAL problem solver. IDEAL problem solver design measured with performance assement consist of IDEAL process and IDEAL product. The average of IDEAL problem solver become value of problem solving skill for every student. The result of the research shows that the implementation of problem based learning increase the students’ problem solving skill that covers the IDEAL problem solver. The total average of IDEAL problem solver in the X-A class SBBS increased from 42,0 move up into 85,7 score. This research’s conclusion is the implementation of problem based learning can increase the problem solving skill of the students in of SMA Negeri Sragen Billingual Boarding School Gemolong academic Year 2013/2014 about enviroment pollution material.</p><p align="center"> </p><p class="0jTULISANKATAKUNCIKEYWORDS">Key Words: Problem based learning, problem solving skill</p>
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