Secretion products and the uptake of material from the outside of the protoplasts of plants and microbes may influence the mutual interaction in host-parasite systems. Organelles involved in secretion by rust fungi have been characterized using immuno-electron microscopy. Proteins with the C-terminal histidine -aspartic acid -glutamic acid -leucine tetrapeptide (HDEL) signal sequence accumulated in tubular vesicular complexes of the ER in the parasitic phase of the fungi. The tubular complexes were differentiated from smooth cisternae that probably correspond to the Golgi equivalent. In the plant, material was secreted into the papilla and at the same time, endocytosis of 1,3-(3-g1ucans by clathrincoated vesicles occurred at the penetration site of the monokaryotic appressorium. During further development of monokaryotic haustoria, different wall layers were produced around the~e haustoria. Components of those layers were plant cell wall constituents most of which were also detected in the Golgi apparatus. One layer was rich in callose, which is supposed to be synthesized at the plasma membrane. Obviously, endo-and exo-cytosis occur permanently by host and parasite during fungal development within the plant.Key words: Uromyces, Golgi, secretion, endocytosis, high-pressure freezing, Saccharomyces, Vigna sinensis, Vicia faba.Resume: La secretions de substances et I'absorption de materiels a partir de I'exterieur de protoplastes de plantes et de microbes peuvent influencer les systemes d'interactions mutuelles entre les hotes et leurs parasites. A I'aide de la microscopie electronique et du marquage immunologique, les auteurs ont caracterise les organeJles impliques dans la secretion de rouilles fongiques. Chez le champignon, au cours de la phase parasitaire, des proteines avec la sequence signaletique HDEL s'accumulent dans Ies complexes tubulo-vesiculaires du reticulum endoplasmique. Les complexes tubulaires se differencient a partir de citernes lisses qui correspondent probablement 11 la structure equivalent 11 I'appareil de Golgi. Chez la plante, au site de penetration par I'appressorium, monocaryote, du materiel est secrete dans les papilles et au meme moment, on observe I'endocytose de 1,3-(3-glucans par des vesicules enveloppees de c1athrine. Au cours des aut res eta pes du developpement de I'hausterie monocaryote, differentes couches parietales sont deposees autour des hausteries. Les composantes de ces couches sont des constituants de parois cellulaires de plantes dont la plupart peuvent egalement etre decelees dans !'appareil de Golgi.Une couche est riche en callose, laquelle est supposement synthetisee sur la membrane plasmique. 11 y a donc des preuves que des endo-et des exo-cytoses sont constamment effectuees par I'hote et le parasite au cours du developpement fongique dans la plante.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.