A glassy carbon electrode modified with electrochemically reduced graphene oxide (ErGO) and poly (3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) was developed for the uric acid determination with dopamine as interference in artificial saliva. The electrochemical performance of the fabricated electrode was studied using both techniques of cyclic voltammetry (CV) and differential pulse voltammetry (DPV), under optimized conditions. Using DPV, the sensor based on ErGO/PEDOT:PSS modified GCE displayed a linear relationship in the concentration ranges of 10-100 µM for uric acid. This uric acid sensor exhibited a high sensitivity with the detection limits of 1.08 µM and the quantitation limit of 3.61 µM. This sensor also showed good reproducibility for uric acid detection in the artificial saliva in 5 consecutive days measurements. This device was successfully used to analyze uric acid in the artificial saliva as well as in human saliva sample using standard addition method.
A molecularly imprinted polymer (MIP) with uric acid cavities increases the selectivity of uric acid measurement in the presence of dopamine as an interferent.
Penelitian ini bertujuan untuk mendeteksi kandungan vitamin B1 (tiamina) pada suplemen vitamin komersial dengan teknik differential pulse voltammetry menggunakan elektrode karbon kaca yang termodifikasi PEDOT:PSS dan membandingkan hasilnya dengan metode spektrofotometri sinar tampak. Investigasi elektrokimia dievaluasi terhadap elektrode karbon kaca termodifikasi PEDOT:PSS dalam parameter kinerja analitik meliputi linearitas, presisi, limit deteksi, dan limit kuantitasi. Berdasarkan hasil studi, puncak oksidasi tiamina terlihat pada potensial sekitar 250 mV vs Ag/AgCl dan intensitas arusnya terukur 2,5 kali lipat lebih besar dibandingkan ketika diukur menggunakan elektrode karbon kaca taktermodifikasi. Selain itu, pengukuran tiamina pada kisaran konsentrasi 1-10 mM menggunakan elektrode karbon kaca termodifikasi PEDOT:PSS menunjukkan nilai yang baik untuk linearitas (R 2 =0,9913), ketelitian (%SBR 3,9%), batas deteksi dan kuantitasi berturut-turut adalah 2,5×10 -5 M dan 7×10 -5 M. Berdasarkan hasil pengukuran tiamina pada sampel suplemen vitamin komersial menggunakan elektrode karbon kaca termodifikasi PEDOT:PSS, nilai ketepatan dapat dihitung sebesar 95,86%. Hasil tersebut dibandingkan dengan nilai sebenarnya dan menunjukkan tidak berbeda signifikan ketika diuji dengan t-student pada selang kepercayaan 95%.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.