Background: A study was conducted to investigate changes in oxidative stress indicators of West African dwarf (WAD) goats at different reproductive stages. There are a total of 35 dry does, 30 gravid does at first trimester (40-45 days post-mating), 33 gravid does at second trimester (85-90 days post-mating), 31 gravid does at third trimester (135-140 days post-mating), and 40 postpartum does (30-35 days postpartum). Fastened blood samples were collected from all tagged animals and serum obtained. Serum was assayed for lipid peroxidation, total antioxidant activity, glutathione peroxidase, catalase and superoxide dismutase, serum biochemical, glucose, total protein, albumin, cholesterol, triglyceride, high-density lipoprotein, alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase, urea, and creatinine using standard procedures. Results: Result revealed that intense metabolic changes were associated with late prepartum and postpartum does as pronounced in oxidative and biochemical indicators of WAD does. Significant differences were found in serum biochemical indices across the different physiological stages of the doe except for triglyceride and low-density lipoprotein. Antioxidant defense is higher at early and mid-gestation and dry does, through low lipid peroxidation and high antioxidant activity as compared to late gestation and lactation does. Conclusion: The observed result shows that the WAD does display oxidative stress during late gestation and lactation owing to intense metabolism. Antioxidant fortification should be employed especially during late gestation and lactation to counteract the oxidative stress in WAD goats.
This work was aimed at evaluating the ability of sodium citrate-egg yolk extender to sustain the viability of spermatozoa in semen stored at room temperature over a period of time as well as determining the best dilution ratio for on-farm artificial insemination. Seven matured West African dwarf bucks were used for this study; semen was collected once weekly with electro ejaculator, maintained at 37 C in the water bath during extension and was evaluated immediately after extension at room temperature until motility dropped below 20%. The semen collected were pooled and divided into 5 portions. Each portion was extended with sodium citrate-egg yolk extender at 1:0 (without extender, control) as T1, (1:1) T2, (1:2) T3, (1:3) T4 and the 5th portion was extended with normal saline at 1:1 (T5) which served as the negative control. Sperm motility, spermatozoa concentration, livability, acrosome integrity and morphological abnormalities were assessed and data obtained were subjected to analysis of variance. Results showed that spermatozoa motility in treatments 1, 2, and 5 differed significantly (P
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.