No abstract

The goal of this study was to investigate the effects of dietary supplementation with b-glucan and microencapsulated probiotics (Bacillus subtilis or Pediococcus acidilactici) on growth performance, body composition, haemolymph constituents, and intestinal morphology and microbiota of the Pacific white shrimp Litopenaeus vannamei. Four treatment diets [basal diet (C), b-glucan-containing diet (b-glu), b-glucan plus B. subtilis-containing diet (b-glu+Bs), and b-glucan plus P. acidilactici-containing diet (b-glu+Pa)] were fed to L. vannamei for 90 days. Shrimp fed the b-glu and b-glu+Pa diets exhibited similar growth performance and body protein content, which were significantly higher than those of shrimp fed the control diet (P < 0.05). No significant differences in haemolymph triglyceride, cholesterol, protein, haemolymph urea nitrogen or chloride were detected among the experimental diets. However, dietary b-glucan alone increased the haemolymph glucose level and osmolarity (P < 0.05). Synbiotic supplementation had greater effects on intestinal microbiota and morphology than dietary b-glucan alone. For example, b-glu+Bs increased the number of intestinal lactic acid bacteria and decreased the number of Vibrio spp. (P < 0.05), and b-glu+Pa increased the height of intestinal villi.