The subcellular distribution of phosphatases, proteinases, and ribonucleases of normal human stratum corneum and psoriatic scales was determined after differential centrifugation. All psoriatic enzymes showed much increased activities as compared to the normal stratum corneum enzymes. The highest activities of alkaline phosphatase from psoriatic scales could be detected in the nuclear fraction. The main activities of all other tested phosphatases and proteinases were present in the cytoplasmatic fraction. The subcellular distribution of the ribonucleases varied according to the pH value.
The specific and total acid phosphatase activities of all fractions of a differential centrifugation of homogenized normal stratum corneum and psoriatic scales were compared. The main enzyme activities of both tissues were localized in the 105,000 g supernatants, i.e. the cytoplasmatic fractions. The kinetic properties of the cytoplasmatic acid phosphatase of both tissues were studied and the influence of several inhibitors investigated. The enzymes in both tissues could be inhibited by p-chloromercuribenzoate, F––, tartrate, Be2+, Cu2+, and Zn2+. Pb2+ only affected the psoriatic enzyme.
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