A nonswarming Serratia liquefaciens mutant deficient in serrawettin W2 production was constructed by transposon mutagenesis. Sequence homology indicated that insertion had occurred in gene swrA, which encodes a putative peptide synthetase. Expression of swrA is controlled by quorum sensing.
SUMMARYThe syntheses of all the possible 2 H , 1 3 C , and 15N singly and multigly labelled isotopomers of acetonitrile and thioacetamide are described. The pathway includes reaction between methyl iodide and potassium cyanide followed by treatment of the acetonitrile with diphenylphosphinodithioic acid to give thioacetamide, purified by sublimation in an overall yield of 55-60%.
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