Diet management is crucial for diabetes to avoid a variety of complications. Due to the large fluctuations in glucose levels that naturally occur over the course of a day, it has been concluded that the biomarker for long-term glycaemic control is whole-blood glycated hemoglobin since its level responds to the long-term progression of diabetes without the short-term fluctuations such as glucose. In this study, an integrated microfluidic chip was developed for measurement of glucose and HbA1c. The whole blood was first separated into plasma and red blood cells (RBC) and then respectively transported into corresponding reaction biosensors with enzyme immobilization. Eventually, both blood glucose and HbA1c levels for short-term and long-term monitoring were measured. The automatic detection system can provide quick monitoring information for the diabetes patients.
A polyphasic taxonomic approach was used to characterize a Gram-stain-negative, orange-coloured bacterium (designated strain CC-SYL272T) isolated from paddy soil. Cells were observed to be strictly aerobic, non-motile and non-spore-forming rods, exhibiting positive catalase and oxidase. Strain CC-SYL272T was found to grow optimally at 20–40 °C, pH 6.0–8.0 and NaCl 0–2 % (w/v). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain CC-SYL272T belongs to the genus
Niabella
, family
Chitinophagaceae
, and is most closely related to
Niabella pedocola
(97.8 %) followed by
Niabella drilacis
(97.2 %) and established a distinct taxonomic lineage associated with these species. The highest orthologous average nucleotide identity (OrthoANI) values were recorded for strain CC-SYL272T
versus Niabella species (69.1–83.5 %, n=8). The mean digital DNA–DNA hybridization (dDDH) value obtained for strain CC-SYL272T against
N. pedocola
was 27.3 %. The polar lipid profile consisted of phosphatidylethanolamine and five unidentified lipids. The major polyamines were putrescine and sym-homospermidine. The dominating cellular fatty acids (>5 %) included iso-C15 : 0, iso-C15 : 1 G, iso-C17 : 0 3OH and C16 : 1
ω6c/C16 : 1
ω7c. The draft genome (6.25 Mb) of strain CC-SYL272T spanned three contigs having 47.1 mol% DNA G+C content, 5087 protein-encoding genes, 10 rRNA genes and 44 tRNA genes. The genome harboured genes involved in the depolymerization of both animal and plant polysaccharides. Based on its distinct phylogenetic, phenotypic and chemotaxonomic traits together with results of comparative 16S rRNA gene sequence, OrthoANI, dDDH and the phylogenomic placement, strain CC-SYL272T is considered to represent a novel species of the genus
Niabella
, affiliated to the family
Chitinophagaceae
, for which the name Niabella agricola sp. nov. is proposed. The type strain is CC-SYL272T (=BCRC 81319T=JCM 34758T).
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.