Assessing the extent to which changes in lacustrine biodiversity are affected by anthropogenic or climatic forces requires extensive palaeolimnological data. We used high-throughput sequencing to generate time-series data encompassing over 2200 years of microbial eukaryotes (protists and Fungi) diversity changes from the sedimentary DNA record of two lakes (Lake Bourget in French Alps and Lake Igaliku in Greenland). From 176 samples, we sequenced a large diversity of microbial eukaryotes, with a total 16 386 operational taxonomic units distributed within 50 phylogenetic groups. Thus, microbial groups, such as Chlorophyta, Dinophyceae, Haptophyceae and Ciliophora, that were not previously considered in lacustrine sediment record analyses appeared to be potential biological markers of trophic status changes. Our data suggest that shifts in relative abundance of extant species, including shifts between rare and abundant taxa, drive ecosystem responses to local and global environmental changes. Community structure shift events were concomitant with major climate variations (more particularly in Lake Igaliku). However, this study shows that the impacts of climatic fluctuations may be overpassed by the high-magnitude eutrophication impacts, as observed in the eutrophicated Lake Bourget. Overall, our data show that DNA preserved in sediment constitutes a precious archive of information on past biodiversity changes.
International audienceTo reconstruct the evolution of livestock in SW Greenland over the last two millennia, we measured the concentration of bile acids in a sedimentary sequence retrieved from Lake Igaliku. Deoxycholic acid (DOC) was the sole bile acid. and was present throughout the sequence.The DOC flux correlated quantitatively with that of coprophilous fungal spores. Maximum DOC and coprophilous fungal spores fluxe was recorded during the two periods of human settlement and grazing activity in the region (i.e. the Norse settlement during the Middle Ages and the recent Danish agricultural phase since 1920). These flux values were consistent with the presence of recent livestock around the lake and are attested to by way of archaeological data relating to the Norse period. In contrast, the DOC and coprophilous fungal spores background during pre-Norse times and the Little Ice Age (LIA), indicated the presence of wild herbivores. Lower DOC and coprophilous fungal spore fluxes values after the Norse abandonment, compared with pre-colonization conditions, could indicate that Norse activity in conjunction with climate pejoration, altered durably the pristine wildlife.Therefore, these quantitative correlations between DOC and coprophilous fungal spores fluxes potentially suggest a quantitative relationship with the livestock grazing in the catchment. The comparison between sedimentary DOC and coprophilous fungal spores provides tremendous highlights on past pastoral dynamics over the last 2000 yr in SW Greenland
International audienceThis paper presents the potential of 5β-stanols and bile acids to act as fecal source biomarkers in order to identify and characterize past agropastoral activities in archaeological and natural archives. First of all, a molecular inventory of 5β-stanols and bile acids was made on fresh fecal human and domestic animal samples, using the same methodology to define the specificity of these molecular biomarkers. The selected species were cow, horse, pig and sheep as they are the major domestic species present in European archaeological sites. To our knowledge, our work constitutes the first report on fecal biomarkers in sheep feces. Bile acids can distinguish diet regime and species having the same diet with greater specificity than 5β-stanols. Fresh human fecal material and historical/archaeological fecal material were analyzed to assess their stability through time by calculating the coprostanol/epi-coprostanol (Cp/epi-Cp) and deoxycholic acid/cholic acid (DOC/C) ratios. Results show that bile acids are more resistant to diagenesis than 5β-stanols, at least on a 700-year time scale. Human and domestic animal fecal molecular imprints were then compared to the molecular content of 65 samples retrieved from archaeological sites, soils and lacustrine sediments to test their ability to trace past land-use dynamics. This study is the first to combine bile acids and 5β-stanols to identify a source of fecal material in lacustrine sediments. The combination of sterols and bile acids can be used in a variety of natural archives and archaeological contexts to define the origin of fecal material, to specify land-use, and to reconstruct past pastoral practices at various spatio-temporal scales
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