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Olfactory cues guide plant parasitic nematodes (PPNs) to their host plants. We tested the hypothesis that non-host plant root volatiles repel PPNs. To achieve this, we compared the olfactory responses of infective juveniles (J2s) of the PPN Meloidogyne incognita to four non-host Asteraceae plants, namely, black-jack (Bidens pilosa), pyrethrum (Chrysanthemum cinerariifolium), marigold (Tagetes minuta), and sweet wormwood (Artemisia annua), traditionally used in sub-Saharan Africa for the management of PPNs. Chemical analysis by coupled gas chromatography–mass spectrometry (GC/MS) combined with random forest analysis, followed by behavioral assays, identified the repellents in the root volatiles of B. pilosa, T. minuta, and A. annua as (E)-β-farnesene and 1,8-cineole, whereas camphor was attractive. In contrast, random forest analysis predicted repellents for C. cinerariifolium and A. annua as β-patchoulene and isopropyl hexadecanoate. Our results suggested that terpenoids generally account for the repellency of non-host Asteraceae plants used in PPN management.

The root-lesion nematode Pratylenchus goodeyi is a common pest of banana in Kenya, which results in significant yield losses. An emerging option for the management of banana nematodes includes the use of endophytic fungi, which cause no damage to their hosts but often extend benefits, such as enhanced protection against various biotic and abiotic constraints. In the current study, non-pathogenic Fusarium oxysporum strains originating from banana {Musa spp.) in Kenya (4MOC321 and 11SR23) and Uganda (V5w2) were evaluated for their efficacy against P. goodeyi in tissue culture-derived plantlets (cv. Giant Cavendish and cv. Grand Nain) in pots. Pratylenchus goodeyi densities were 47-60% lower in endophyte-inoculated relative to non-inoculated plants and root necrosis damage was reduced by >30%. Endophyte treatment had no effect on plant growth parameters compared with untreated control plants. The performance of the two Kenyan strains was equally effective as the positive control (V5w2), thus demonstrating their potential as nematode bio-control agents under Kenyan growing conditions.

Fruit quality is preserved through cold storage, but climacteric fruits are prone to chilling injury (CI) which limits their shelf life and marketability. Two postharvest treatments, 1 mM methyl jasmonate (MeJA) and 4% (wt/vol) calcium chloride (Ca2+), were separately used to investigate their influences on chilling injury (CI) incidence and fruit quality in unpacked banana cultivar “Grand Nain” during cold storage and subsequent ripening. Banana fruits were dipped for 2 min in aqueous emulsions containing 1% Tween‐80—used here as a surfactant with untreated fruits being used as control. Fruits were stored at 10 ± 2 or optimal 14 ± 2°C temperature and relative humidity 85%–90% for a 20‐day cold storage period and then removed from cold storage at 5, 10, 15, and 20 days followed by ripening at 22 ± 2°C. Treatments with MeJA or Ca2+ significantly reduced CI in banana fruit during cold storage and subsequent ripening temperature. Untreated controls exhibited increased CI, weight loss, and decreased hue angle, as well as firmness. In contrast, the aforementioned changes were considerably delayed after treatments with MeJA or Ca2+. Application of MeJA or Ca2+ also increased total phenolic compound contents and maintenance of total antioxidant activity throughout cold storage and during ripening periods as compared to that of the control. These findings indicate that coating bananas with 1 mM MeJA or 4% (wt/vol) Ca2+ can improve the postharvest quality and shelf life of fruits, and it can ameliorate chilling injury during cold storage and at ripening temperature.