In recent years, there has clearly been a trend towards widespread dissemination of scientific results and achievements by sharing them in the form of educational materials. The application of the results of a research project in the education and knowledge development of students, young researchers and scientists, brings an added value to the outcomes of the scientific project. This work aims at contributing to the global process of free access to scientific materials by sharing the experience of a research group from the Medical University of Varna (MUV) in systematizing the results of three research projects, their transformation into educational resources and their application in innovative courses. The consolidated and systematized results of a research project are valuable assets for development of innovative educational materials. These resources, uploaded in a shared online repository, contribute to strengthen the international scientific community networking.
Observation of complex cellular shapes in three-dimensional (3D) tissue context requires the use of thick blocks of tissue samples, which are not transparent. To overcome this optical opacity, a specific set of histological techniques have been developed and named tissue clearing methods. Here we review the major available tissue clearing protocols and justify the advantages of a particular protocol of tissue clearing named CUBIC over the other methods in imaging and 3D reconstruction.
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