The fluorescence anisotropy decay dynamics of the fluorescent probe Coumarin‐153 (C153) have been investigated in two neutral micelles, Triton‐X‐100 (TX‐100) and Brij‐35 (BJ‐39, at different temperatures and analyzed on the basis of the wellknown two‐step model. Because steady‐state fluorescence spectra of the above probe do not show any noticeable changes with respect to temperature, for either of the studied micelles, suggests a similar polarity in the microenvironment around the probe at all the temperatures studied. The anisotropy results indicated that, for both the micelles, the fluidity inside the Palisade layer increases with temperature. However, the temperature effect on the anisotropy decay is relatively more pronounced in TX‐100 than in BJ‐35. It is inferred that the temperature effect on the anisotropy decay in the BJ‐35 micelle is mainly due to the thermal effect on the microviscosity in the micellar phase. In the case of TX‐100, the results indicate that, along with the above thermal effect, an additional effect is observed due to the increased size and hydration of the micelle with temperature, with the result being that the fluorescence anisotropy decay in TX‐100 is more sensitive to temperature than in BJ‐35. In the TX‐100 micelle, our studies show that with an increase in temperature, even though the micellar size increases substantially, the distance of the probe from the micellar core does not increase that significantly. Thus, with increasing temperature, the probe undergoes a relative migration toward the micellar core to avoid the increased hydration in the micellar Palisade layer.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.