Surface functionalization has attracted
considerable interest from
researchers because of its capability for facilitating the interface
communication between an energy transducer and a biological system.
We report newly synthesized N-(4-aminobenzoyl)-N′-(4-maleimidobenzoyl)-1,2-ethylenediamine (AME)
to promote the modification of the surface with thiolated DNA and
3-((4-aminophenyl)dimethylammonio)propane-1-sulfonate (APSB) for the
facile formation of a bifunctional interface forming an antifouling
surface. Through the formation of diazonium ion, electrochemically
reductive deposition of the two arylamines can fabricate simultaneously
and effectively a bifunctional surface on a gold electrode and install
a DNA probe to form a sensor; the sensor was applied to detect three
genetic fragments of the New Delhi Metallo-β-Lactamase (NDM)-coding
gene. Effects of the diazotization and the conditions of electrochemical
deposition upon the sensing signals were investigated in connection
with enumerating the accessible maleimide groups and assessing the
diffusion resistance of the electroactive indicator. The detection
limit given by the diazonium-constructed system was improved to attain
the level of 54 pM, an advance over a conventional self-assembled
monolayer.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.