Key WordsActiniceps thwaitesii; Anastrophella podocarpicola; new record; new species.During recent surveys of litter-decomposing basidiomycetes in Japan, two minute species were collected and their multisporous isolates were obtained. These species are described and illustrated with their cultural features.
Materials and MethodsDescriptions of macroscopic and microscopic features are based on dried specimens. Dried basidiomata were rinsed with 70% ethanol, then transferred to distilled water for rehydration. Rehydrated basidiomata were mounted for micromorphological observation in distilled water, 3~ KOH plus Congo red and phloxine, or Melzer's reagent. Color terms and notations are taken from Kornerup and Wanscher (1978). Spore statistics included: R, the arithmetic mean of the spore length and spore width (-+SD) for n spores measured; Q, the quotient of spore length and spore width in any one spore, indicated as a range of variation in n spores measured; and Q, the mean of Q-values (-+SD).Spore deposits were obtained on potato dextrose agar (PDA; Nissui, Tokyo) and subsequent multisporous cultures were isolated. Cultures were grown on PDA and malt extract agar (MEA) (Nobles, 1965). The Nobles species code (Nobles, 1965) was recorded for 4-wkold cultures on PDA. Extracellular oxidase reactions were tested on 4-wk-old cultures on PDA according to Desjardin (1990 Figs. 1, 2, 5 Basidiomata 0.3-0.6 mm tall, stipitate-capitate, white. Capitula 0.1-0.2 mm diam, globose at maturity. Stipe 0.2-0.3x 0.02-0.05 mm, cylindrical, insititious. Basidiospores 6.6-9.4 x 3.3-4.6 #m (~= 7.8-+0.8 • 3.9--+ 0.4#m; Q=1.7-2.8; Q=2.0+0.3; n-20), oblong to subcylindrical, smooth, hyaline, thin-walled, inamyloid. Basidia 19-30x 5-7 #m, narrowly clavate, 2-spored; sterigmata 4.0-4.5#m long. Cystidia 47-115• #m, conical, thickly encrusted, projecting up to 40#m beyond hymenial surface, often secondary septate, thick-walled up to 1.5 #m, hyaline, inamyloid; crystals very slowly soluble in 3~ KOH. Acanthophyses 20-44 • 4-7 f~m, narrowly clavate, often slightly encrusted, densely beset with outgrowths, projecting 12-20 #m beyond hymenial surface, thin-to thick-walled up to 1.5#m, hyaline, inamyloid; outgrowths up to 2.5#m long, 0.5-2.0#m wide. Stipe hyphae 2-4#m diam, glassy, parallel, tubular, smooth, thick-walled up to 1.5 #m, hyaline, irregularly turgescent in 3~ KOH, dextrinoid. Caulocystidia absent. Clamp connections absent.Cultural features. Macromorphology on PDA: Mean radius of colony at 23~ is 20 mm, 1 wk (n=4); 31 mm, 2wk; 40mm, 3wk; over 45ram, 4wk. Advancing zone appressed. Aerial mycelium wooly, dense, white. Reverse yellowish white (3A2). Odor indistinct. Macromorphology on MEA: Mean radius of colony at 23~ is 12ram, lwk (n--4); 23mm, 2wk; 34mm, 3wk; 42 mm, 4 wk. Aerial mycelium wooly, sparse, white. Reverse white. Odor indistinct. Micromorphology on