Little is known about the presence of common medical pathogens in the human oral cavity. Using a 16S rRNA-based PCR identification method, this study determined the occurrence of Porphyromonas asaccharolytica, Bacteroides fragilis and Chlamydia pneumoniae in subgingival plaque from 50 adults with advanced periodontitis. Each patient contributed samples from 3 deep periodontal pockets collected by paper points. The PCR primers were for P. asaccharolytica 5'-CTC TAG CTA GAG TGT ACT GG-3' and 5'-ATA GGG TTT ATA GAT TAG CTC TCT-3', for B. fragilis 5'-AAT GAT TCC GCA TGG TTT CAT TA-3' and 5'-GCG GTG ATT GCT CAC TGA CA-3', and for C. pneumoniae 5'- TGA CAA CTG TAG AAA TAC AGC-3' and 5'-CGC CTC TCT CCT ATA AAT-3'. The primers yielded a single amplicon with the respective reference strains and produced no amplicon with colonies of 25 groups of oral organisms. None of the three test species were detected in any of the 50 pooled subgingival samples tested. P. asaccharyolytica, B. fragilis and C. pneumoniae do not seem to be part of the periodontopathic microbiota in humans.
Porphyromonas endodontalis is a relatively frequent isolate AAC GTA TTC ACC-3), which match nearly all bacterial 16S rRNA sequences at the same position (base positions 786-808 from dental periapical abscesses of endodontal origin [1]. Most organisms in endodontic lesions originate from supragingival and and 1,369-1,387, respectively; Escherichia coli nomenclature) but not 18S rRNA sequences of eukaryotic cells, served as the positive subgingival dental plaque. P. endodontalis has been recovered from the tonsillar area and the dorsum of the tongues of patients control for the PCR. The primers were synthesized on a Beckman automated DNA synthesizer (Beckman Instruments, Fullerton, with periodontitis [2], but the organism's primary oral ecological niche has not been identified. P. endodontalis, in contrast to Por-CA). PCR was performed directly from VMGA III specimens (direct phyromonas gingivalis, does not produce a trypsin-like enzyme and does not exhibit hemagglutination activity, but most conven-specimen PCR) or from colonies collected after culture of specimens (culture-plate PCR). The PCR was performed essentially as tional microbiological tests do not readily distinguish between P. endodontalis and some other black-pigmented gram-negative described previously [3]. The amplification took place at 95ЊC for 2 minutes, 36 cycles of 94ЊC for 30 seconds, 60ЊC for 1 minute, anaerobic rods [1]. However, a 16S rRNA-based PCR identification method may be capable of detecting P. endodontalis, as it has and 72ЊC for 2 minutes, followed by 72ЊC for 10 minutes. Amplification products were analyzed by electrophoresis in a 1% agarose other oral anaerobic gram-negative species [3]. In the present study, we determined the occurrence of P. endodontalis in ad-gel with ethidium bromide. The P. endodontalis primers yielded a single amplicon with vanced periodontal lesions with use of PCR.We collected 50 subgingival samples from 27 females and 23 P. endodontalis colonies and produced no amplicon with colonies of 23 other subgingival organisms (table 1). The ubiquitous posi-males with periodontitis. The patients' ages ranged from 15 years to 73 years (mean age, 50 years). Samples were collected by paper-tive control primers demonstrated the predicted amplicon in each experiment performed. points in VMGA (viability medium Gothenberg anaerobic) III transport medium [4]. Each specimen included material from three P. endodontalis was not detected among the predominant cultivable microbiota in any sample. However, when PCR was per-deep periodontal pockets. All samples were processed within 16-48 hours of collection. Quality assurance tests revealed that inocu-formed on pooled colonies from culture plates with heavy growth, the P. endodontalis-specific amplicon appeared in 22 samples lated P. endodontalis survived in VMGA III for at least 3 days. The samples were diluted 10-fold in VMG I [4] and plated on (44%; table 2). PCR of the direct specimens revealed the P. endodontalis-specific amplicon in 33 of the 50 samples studied (66%; 4.3% ...
Background: Smile aesthetics has a vital role to play in an individual’s life and one of the factors affecting the beauty of the smile is gingival color. A gingival color change or gingival hyperpigmentation causes an unesthetic smile line, especially in patients with a gummy smile, which is also known as a black gummy smile. Numerous gingival depigmentation methods have been performed successfully for ablating gingival melanin pigmented epithelium. Thus, the aim of this study is to evaluate the treatment efficacy of gingival hyperpigmentation by using a carbon dioxide (CO2) laser. Methods: A cross-sectional descriptive study was carried out with 38 patients at a hospital in Vietnam. Ponnaiyan classification and the Hedin melanin index were used to assess the distribution and extent of gingival pigmentation in the study. Pain assessment was performed using the Visual Analog Scale (VAS) to evaluate the intensity of pain during the laser treatment. In addition, clinical evaluation (i.e., wound healing) of each treatment procedure was conducted using the three level Dummett–Gupta Oral Pigmentation Index (DOPI) assessment. Results: This study showed that less pain was experienced by patients treated by CO2 laser; the rates of no pain, mild pain and moderate pain after treatment were, respectively, 21%, 76% and 2.6%; there was 100% complete epithelization after 1 week. The DOPI rates for turning from a DOPI score of 1, 2 or 3 to a DOPI score of 0 after a 12-week treatment were 87.5%, 76.9% and 24%, respectively. Conclusions: Using a CO2 laser for gingival melanin pigmentation treatment is a safe and effective procedure.
Abstract. The objective of this study is to determine the correlation between periodontitis and obesity in patients reporting at the Tradi-tional Medicine Institute in Ho Chi Minh City, Vietnam. This cross-sectional study sample consisted of 679 adult patients aged between 18 and 83 years who visited examination department, the Traditional Medicine Institute, Ho Chi Minh City, Vietnam. All participants completed the questionnaire, had anthropometric (weight, height, waist circumference) and body fat percentage measurements; and periodontal examination (BOP, PI, GI, PD and CAL). The fasting glycemic level and type 2 diabetic status were also determined. Whatever different definitions of obesity used (body mass index, waist-hip ratio and body fat per-centage), the prevalence of periodontitis in the obese group was significantly higher when compared with the non-obese group (p <0.05). In multivariate logistic regression analysis, body mass index, waist-hip ratio and body fat percentage defined obesity were significantly associated with periodontitis, presenting OR of 2.16 (1.35-3.45), 2.09 (1.34-3.26), and 2.71 (1.82-4.05), respectively (p<0.05). BOP and CAL were also significantly correlated with periodontitis. Conclusion: Obesity was significantly associated with increased odds of periodontitis (p<0.05).
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.