Although pseudorabies virus can affect a wide range of mammalian and avian hosts, swine are the only natural hosts of the virus. The US commercial swine industry obtained pseudorabies-free status in 2004, which was important because of the economic value of domestic swine production; however, feral swine remain competent hosts and represent a constant threat for reintroducing the virus into the commercial industry. To better assess feral swine infection status, we collected 8,498 serum samples from feral swine across the United States between 1 October 2009 and 30 September 2012. Of these, 18% were antibody positive in 25 of 35 states where samples were collected, indicating that transmission risk is widespread.
Pseudorabies virus (PRV) is the cause of Aujeszky's disease, a disease that is significant economically for the swine industry worldwide. A real-time polymerase chain reaction (PCR) assay based on the gB and gE genes was used to identify PRV nucleic acid in diagnostic samples. Using virus isolation (VI) as the gold standard, the PCR assay performed well in a variety of diagnostic matrices. Testing was conducted on 1,027 nasal swabs with the following findings:
Despite successful eradication of pseudorabies virus (PRV) from the commercial pig industry in the United States in 2004, large populations of feral swine in certain regions act as wildlife reservoirs for the virus. Given the threat of reintroduction of the virus into domestic herds, a rapid, reliable, easily implemented assay is needed for detection of PRV. Although a real-time PCR (rtPCR) assay exists, improvements in rtPCR technology and a greater understanding of the diversity of PRV strains worldwide require an assay that would be easier to implement, more cost effective, and more specific. We developed a single-tube, rapid rtPCR that is capable of detecting 10 copies of PRV glycoprotein B ( gB) DNA per 20-µL total volume reaction. The assay did not produce a false-positive in samples known to be negative for the virus. The assay was negative for genetically similar herpesviruses and other porcine viruses. Our assay is a highly specific and sensitive assay that is also highly repeatable and reproducible. The assay should be a useful tool for early detection of PRV in pigs in the case of a suspected introduction or outbreak situation.
The transmission of disease among livestock farms could be addressed by the efficiency of truck washes to clean and disinfect trailers used for transporting animals. Collecting swab samples from trailers and cabs of identified truck wash trailers will help to determine the proper procedures and steps needed to reduce the transmission of disease. Truck washes in the state of Iowa were identified and invited to participate in a questionnaire that will provide helpful information for this research. The main goals of this study are to 1) determine the areas in the truck washing process that pose a high risk to transmit disease, and 2) to identify the location of current livestock truck washes and their capability in the event that some disease outbreak requires their involvement. This survey tool could help to provide necessary information in order to determine which service methods are best for reducing back contamination and the spread of disease among livestock herds. Determining what locations would be beneficial in collecting samples will be easier overall when the surveys are completed.
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