Abstract. Tanjung A, Saragih HTSSG, Trijoko, Soenarwan HP, Widianto S, Mahardhika IWS, Daryono BS. 2019. Polymorphism of myostatin gene and its association with body weight traits in a hybrid of GAMA chicken (Gallus gallus domesticus Linn. 1758). Biodiversitas 20: 3207-3212. An experiment was conducted to detect SNP of the myostatin gene and its association with the body weight of hybrid chicken crossbreed from F1 Kamper and BC1 Broiler. Four F1 Kamper hens were crossbred with BC1 Broiler rooster. Day old chick (DOC) hatched were maintained for 49 days with body weight measurement every seven days. The blood samples from 49 days old chicken were taken for DNA isolation by Chelex 5% method and then amplification of the myostatin gene. PCR products were sequenced, and sequence alignment was performed using Clustal Omega to obtain SNP. The SNP obtained was analyzed by the Pearson correlation test with the body weight of forty nine-days-old chickens. The body weight of the hybrid chicken is higher than of Pelung chicken but lower than the Broiler. There are 7 SNPs in myostatin gene exons included 2 Adenine insertions, 1 Guanine deletion, and four substitutions (C2244G, G2283A, T4842G, G7378T) that yield nine haplotypes. Six haplotypes had different protein sequences with Myostatin protein, while three haplotypes were identical to Myostatin protein. The correlation analysis showed that there was a strong positive correlation (r = 0.736) between normal Myostatin protein and mutant to chicken body weight at 49-days-old. Adenine insertion to nucleotide 2099-2100 of myostatin gene had a very strong positive correlation (r = 0.800) to 49-days-old chicken body weight, although T4842G substitution had a strong negative relationship (r = -0.773) to 49-days-old chicken body weight. Adenine insertion to nucleotide 2099-2100 of myostatin gene could be a genetic marker of heavier body weight of the hybrid chicken.
Abstract. Giant tiger shrimp (Penaeus monodon Fabricius, 1798) is one of Indonesia's main aquaculture commodities. Four populations (G7, G8, G8iA and G8iJ) of tiger shrimp were obtained from broodstock center of Main Center for Brackishwater Aquaculture and Fisheries (MCBAF) Jepara, Central Java, Indonesia. G7 is the domesticated stock in MCBAF Jepara. Inbreed progeny was obtained from sibling mating of G7. Outbreed progeny was obtained from mating G7 with wild broodstock. Ten individuals of each population were evaluated for their genetic variation using ISSR marker. The following genetic variation parameters including Percentage of Polymorphic Bands for G7, G8, G8iA and G8iJ were 83.33%, 69.23%, 84.62%, and 72.00% respectively. Expected Heterozygosity values ranging from 0.235 (G8iJ) to 0.322 (G8iA). Shannon's Information Index ranged from 0.317 (G8) to 0.432 (G8iA). A number of Different Alleles was ranged from 1.536 (G8iJ) to 1.714 (G8iA). A number of Effective Alleles ranged from 1.405 (G8iJ) to 1.571 (G8iA). Outbreed population has higher genetic variation than inbreed population.
Sea urchin (Echinoidea) is an avertebrate animal whose habitat can be found from the intertidal to shallow subtidal areas. Sea urchin has a very important role in reef ecology, especially in intertidal and subtidal areas. Sea urchin gonad also can be consumed and has high economic value. The aim of this study was to determine the profile of fatty acid Sea urchin gonad in South Coral in Gunung Kidul (Daerah Istimewa Yogokarta) Gonad samples were taken from two sampling locations, those were four species from Sepanjang beach and one species from Wediombo coast. Fat was separated from the gonad using the method of Blight and Dyer (1959) and fatty acid methyl ester were prepared by direct transesterification reaction according to Morisson and Smith’s method (1964). Fatty acid methyl ester were separated and analysed by gas chromatography. The result showed that there are 10 types of fatty acid found in sea urchin gonad belonging to saturated and unsaturated fatty acid both MUFA (monounsaturated fatty acid) dan PUFA (polyunsaturated fatty acid). In all samples. The level of saturated fatty acids is higher than the unsaturated fatty acids, especially myristic (C14:0) and palmitic acid (C16:0).Keywords: Fatty acid, Sea Uechin, South Coral Gunung Kidul Daerah Istimewa YogyakartaAbstrakLandak laut (Echinodea) merupakan hewan avertebrata yang banyak dijumpai pada daerah pasang surut yang berbatu dan berpasir. Landak laut memiliki peranan yang sangat penting pada ekologi karang terutama di daerah pasang surut, selain itu gonadnya juga dapat dikonsumsi dan memiliki nilai ekonomi yang tinggi. Tujuan dari penelitian ini mengetahui keragaman spesies dan komposisi asam lemak gonad Landak laut yang ada di Pantai Selatan Kabupaten Gunung Kidul. Sample gonad diambil dari 2 lokasi sampling yaitu empat jenis dari Pantai Sepanjang dan satu jenis dari Pantai Wediombo. Lemak dipisahkan dari gonad menggunakan metode Blight and Dyer (1959) dan dimetilasi melalui metode Morison and Smith (1964). Kadar asam lemak gonad kemudian dianalisa dengan GC. Hasil analisa asam lemak sampel gonad ditemukan Asam lemak jenuh yang meliputi asam kaprilat (C8:0), asam miristat (C14:0), asam palmitat (C16:0), asam stearat (C18:0) dan asam arakhidat (C20:0). Asam lemak tidak jenuh antara lain asam oleat (C18:1n-9), asam palmitoleat (C16:1n-7), linoleat (C18:2n-8) dan asam eicosapentaenoat (C20: 5n-3). Hasil analisa menunjukkan bahwa kadar asam lemak jenuh lebih tinggi dibanding asam lemak tidak jenuh terutama asam miristat sebesar 27,20% dan palmitat 24,44% sedangkan asam lemak tak jenuh yang tinggi adalah asam Eicosapentaenoat sebesar 14,83%, keduanya ditemukan pada Colobocentrotus sp.2. Jenis Landak laut di Pantai Selatan Kabupaten Gunung Kidul sangat beragam sedangkan jenis asam lemak yang terkandung pada lima sampel gonadnya sama tetapi berbeda kadarnya.Kata kunci: Asam lemak, landak laut, karang Gunung Kidul Daerah Istimewa Yogyakarta
Background Pelung chicken has extensively been studied through selective breeding and used by the local poultry sector for ornamental purposes and occasionally as meat-type chicken. However, a well-documented and detailed description of its origins, genealogical backgrounds, unique traits, and diagnostic genotyping of its unique plumage colouration has never been compiled. Therefore, this study aimed to provide a detailed description of Pelung chicken and conduct a diagnostic genotyping of the TYR gene associated with golden plumage colouration accompanied with direct visual observations in Pelung chicken. Results Direct visual observations of GK resulted in dominant white shank governs by two autosomal loci and one sex-linked locus. Plumage colours were divided into four variants: black-brown barred, brown-golden barred, brown, and white. Each plumage colour group governs either by both the autosomal loci and recessive sex-linked locus or only autosomal locus. The diagnostic genotyping detected the presence of intron 4 retroviral sequence insertional mutation of tyrosinase (TYR) gene in both F1 Kamper and GK. Full-length retroviral insertional mutation of the G. gallus TYR gene is associated with the appearance of recessive white (C*C/C*C) chickens, with pigmented eyes. Conclusions The golden Pelung chicken was originated from inbreeding crossings between F1 Kamper, the progenies of crossbreeding between Layer Lohmann Brown-Classic and Pelung chicken. Historical accounts suggested the first documentation about the possible origin of Pelung chicken located in several villages surrounding Warungkondang, Cianjur, West Java. Genealogical background of Pelung chicken referred to Thailand RJF (G. g. gallus) and the possible contribution of Javanese fowl (G. g. bankiva) to the formation of domesticated chicken breeds. The genealogical background resulted in two different taxonomical terms, G. gallus and G. gallus domesticus as a subspecies of RJF. The unique and distinctive characteristics of Pelung chicken are crowing duration and plumage colour composition. Our findings provide essential information to assist the development of MAS and conservation initiative of the Pelung chicken germplasm. Our findings provide essential information for modern chicken breeders to assist the development of MAS and conservation initiative of the Pelung chicken germplasm.
In Indonesia, research for the prime seed of Macrobrachium rosenbergii crossbreeding is rarely done. The aims of this study are to study F1's morphological character and genetic diversity from the result of crossbreeding between M.rosenbergii Samas, Bone, and Sintetis populations. In this study, genetic characterization was known by using PCR RAPD method utilizes three primers and morphological characterization. Data were analysed with UPGMA algorithm and Simple Matching coefficient that were presented in dendrogram. F1's coefficient heterosis was counted based on ratio from the average of cephalothorax and abdoment lenght and also ratio from the average of standard length of charapax and the average of abdomen length. The results showed that there was a very high genetic diversity in F1 population. The specific locus was found in individuals from Sintetis and Samsam populations.100% Polimorfism was found from F1's DNA amplification result, meanwhile monomorphism 50% was found from parent's DNA amplification result on OPA 20. The highest F1 heterosis was found in Samas and Sintetis genotipe.
Pituitary Positive Transcription Factor-1 gene is closely related to chicken growth and productivity. This research was conducted to detect Single Nucleotide Polymorphism in the exon 6 Pituitary Positive Transcription Factor-1 gene and its association with the bodyweight growth in the first backcross hybrid chicken. Procedures of the research included crossbreeding female first filial broiler chicken with male Pelung chicken to obtain first backcross hybrid chicken, Day Old chick hatched were maintained during 49 days, the bodyweight on the Day-Old chick measured every seven days, DNA was isolated by Chelex 5% method, Pituitary Positive Transcription Factor-1 gene was amplified by PCR, DNA band was visualized utilizing electrophoresis, and the PCR product was sequenced using Sanger method. The DNA sequence was aligned using Clustal omega software to gain Single Nucleotide Polymorphism. The Single Nucleotide Polymorphism was analyzed using the Pearson correlation test between chicken body weights of 49-days-old chickens with the polymorphism points. The conclusion indicated that the bodyweight of the first backcross hybrid chicken was higher than the Pelung chicken but lower than the first filial broiler chicken. Single Nucleotide Polymorphism was not found on the exon 6 Pituitary Positive Transcription Factor-1 gene in the first backcross hybrid chicken.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.