Many commercialized synthetic antioxidants are used under strict regulations in certain countries because of their potential health hazards. Thus, the search for alternative antioxidants from natural products Indonesia is needed, and one of the potential material is from seaweed extract. Red seaweed is very potential to be developed as a raw material for medicines and cosmetics because it has antioxidant activity, which from its phenolic compound that can remove the free radicals and can inhibit tyrosinase enzyme activity. The purpose of this research is to know the species of red seaweed in the East Nusa Tenggara sea, its total phenolic content, antioxidant activity, and also inhibitory activity of tyrosinase in red seaweed. conduct the research. Parameters that measured are the number of total phenolic compounds, IC50 that obtain from the DPPH test, and inhibition percentage of Tyrosinase. The result shows that there are ten species of red algae in Kupang, East Nusa Tenggara, and it consists of 4 genera. Some of that red seaweed species are potential as antioxidant and tyrosinase inhibitors source. The result from total phenolic content analysis show the species that have highest total phenolic content is Laurencia sp. (24.97 mg GAE.g-1), the antioxidant activity test show the species that have highest antioxidant activity is Gelidium latifolium (46.68%), and the species that have the highest percentage inhibition of tyrosinase enzyme is Gracillaria foliifera (25.21%). Moreover, the research also shows a strong correlation between the total phenolic content of the seaweed extract with antioxidant activity. A post-publication change was made to this article on 18 Jun 2020 to correct the title and an author name.
Penelitian bertujuan mempelajari respon pemberian asam humat pada beberapa macam kombinasi medium tumbuh terhadap kandungan senyawa aktif pada tanaman Pegagan (Centella asiatica L. Urb). Melalui pemberian pupuk asam humat pada kombinasi medium diharapkan pertumbuhan dan metabolism tanaman meningkat yang kemudian berdampak pada meningkatnya senyawa aktif hasil metabolit primer. Medium yang digunakan adalah : tanah & pasir (1 : 1), tanah & sekam (1 : 1), dan tanah & zeolite (1 : 2). Asam humat yang diperlakukan ada 4 konsentrasi, yaitu : 0 gr/kg medium, 4 gr/kg medium, 8 gr/kg medium dan 12 gr/kg medium. Metode penelitian yang digunakan adalah eksperimen menggunakan Rancangan Acak Lengkap dengan pola perlakuan factorial. Sebagai Faktor Utama adalah kombinasi medium, dan sebagai Faktor Kedua adalah konsentrasi asam humat. Parameter yang diamati adalah kandungan alkaloid, flavonoid, dan terpenoid. Hasil penelitian menunjukkan bahwa medium tanam tanah dengan pasir dengan penambahan asam humat 8 gr/kg medium efektif dalam meningkatkan kandungan alkaloid dan biomassa tanaman pegagan. Sedangkan kandungan flavonoid tertinggi diperoleh pada medium tanah dan zeolite dengan penambahan asam humat 4gr/kg medium. Teridentifikasi adanya senyawa terpenoid dengan adanya cincin berwarna coklat pada ekstrak yang dilarutkan dalam chloroform , asam asetat dan asam sulfat pekat.
A study on the growth of plbs of intergeneric hybrids between Phalaenopsis 2166 and Vanda ‘saint valentine’ on four different in vitro culture media was conducted. This study was aimed to find out the best growth medium for intergeneric hybrids between Phalaenopsis 2166 and Vanda ‘saint valentine’, and to obtain medium producing the best roots for the hybrids. The media used were MS, NP, VW, and Knudson. The results showed that all four in vitro culture media were suitable for the hybrid’s growth. In addition, medium NP (New Phalaenopsis) produced the best and most extended root length. Hence, it is recommended to use the media for growing plbs of the intergeneric hybrids between Phalaenopsis 2166 and Vanda ‘saint valentine’ before acclimatization.
In this study, the molecular profiles of five soybean cultivars (Burangrang, Gema, Grobogan, Panderman, and Sinabung) exhibiting salinity resistance were elucidated. The DNA profiles of the five cultivars were found to differ based on simple sequence repeat (SSR), insertion-deletion polymorphism (InDel)-QS080465, and sequence characterized amplified region (SCAR)-QS08064 markers. Three distinct SSR profiles¾Satt-243, Satt-294, and Satt-308¾and the SCAR-QS08064 marker were only observed in the Grobogan cultivar, whereas the InDel-QS080465 marker was only successfully amplified from the Burangrang, Gema, and Grobogan cultivars. The results indicate that the Grobogan cultivar is the most tolerant soybean cultivar, followed by the Burangrang and Gema cultivars. Results were consistent with those from genetic similarity analysis, which showed that Grobogan is genetically more similar to Burangrang and Gema compared to Sinabung and Panderman. In conclusion, the five soybean cultivars have different molecular profiles that are related to their resistance to salinity. SSR markers, InDel QS080465-152, and SCAR QS08064-383 are molecular markers specific to salinity-resistant cultivars.
The improvement of gogo rice production in dryland is constrained by its extraordinary height, which leads to falling, and its long lifespan. The shorter gogo rice plant can be obtained through growth inhibition using paclobutrazol. Gogo rice plant var. Inpago Unsoed 1 was used during the study. An experimental study was performed using factorial completely randomized design. The main factor was four paclobutrazol concentrations (0,100, 200, and 300 ppm). The sub-main factor was paclobutrazol application time after planting (three, four, and five weeks). The results showed that the interaction between paclobutrazol concentration and application time was highly significantly affecting plant height. The most appropriate treatment resulting in optimum plant height was the combination of 100 ppm paclobutrazol applied four weeks after planting. Interaction between paclobutrazol concentration and application time also significantly affect stem diameter, leaf width, and stomata number in the lower leaf epidermis. However, there was no significant effect of paclobutrazol observed on stomata size. The significant result of the combination of paclobutrazol concentration and the application time was also supporting tissue thickness. The thickest tissue was obtained by 300 ppm of paclobutrazol applied five weeks after plantation.
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