The lytic outcome of natural infection by Chlamydia trachomatis was exploited to select CHO (Chinese hamster ovary) cells, following chemical mutagenesis, that were deficient in their ability to sustain productive chlamydial infection. Four distinct mutant cell phenotypes with defects in either attachment or postattachment mechanisms that are required for infection by C. trachomatis and Chlamydia pneumoniae were characterized.
The strain used in these experiments was determined by sequencing of the ompA gene to be C. psittaci (6BC-type) strain (6BC PF ). To validate the retrospective identification of the strain used in these studies as C. psittaci, the results were repeated with concurrently sequence-verified C. psittaci. The data published are accurate for each cell line, but the strain designation was not correct (see corrected Fig. 1 on following page). The use of C. psittaci rather than C. pneumoniae does not fundamentally change the conclusions of the study, as the purpose of using C. pneumoniae was to employ a strain that was phylogenetically distant from C. trachomatis, thereby permitting broad characterization of the mutant cell phenotypes. The use of C. psittaci fulfills this aim by demonstrating the spectrum of attachment and uptake deficiencies and supports the key conclusion that attachment and uptake involve separate molecular mechanisms specific for host cells.Although infectivity of the mutant cell line by C. pneumoniae cannot be addressed because of the nascent resistance to infection of the parental CHO-K1 line, we discovered that sequence-confirmed C. pneumoniae attached to CHO-K1 cells. CHO-6, a mutant cell line resistant to attachment by C. trachomatis and C. psittaci, was tested and was found to be resistant to attachment by C. pneumoniae (corrected Fig. 2 on following page). Other mutagenized CHO cell lines that were determined to have an attachment defect, such as CHO-17, CHO-30, and CHO-5, were tested and also found to be resistant to attachment by C. pneumoniae (data not shown). We can conclude that the phenotypes of these mutagenized cell lines reflect at least one cellular component required in common for attachment of C. trachomatis, C. psittaci, and C. pneumoniae that is separate from postadherence processes ultimately leading to productive infection. Continued on following page1996
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