Adhesion of platelets to the exposed extracellular matrix proteins at sites of vascular injury is partly regulated by the local £uid shear stress. Because the Leu33Pro (Pl A ) polymorphism of integrin L L 3 confers only a modest increase in adhesion under static conditions, we used CHO and 293 cells expressing the Leu33 or Pro33 isoform of L L 3 in £ow chamber experiments to test whether shear forces would alter the Pl A adhesive phenotype. We found that shear force augmented the Pro33-mediated enhanced adhesion to ¢brinogen. This Pro33-dependent enhancement was aspirin-sensitive and was also observed on immobilized von Willebrand factor and cryoprecipitate, but not ¢bronectin. Thus, shear stress enhances the adhesive phenotype of the Pro33 cells to multiple physiologic substrates. ß
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