To understand the genetic diversity and differentiation of Vietnamese melon (
Cucumis melo
L.), we collected 64 landraces from the central and southern parts of the country and assessed molecular polymorphism using simple sequence repeat and random amplified polymorphic DNA markers. The Vietnamese melon was divided into seven cultivar groups, namely “Dua le”, “Dua vang”, “Dua bo”, “Dua gang-andromonoecious”, “Dua gang-monoecious”, “Dua thom”, “Montok”, and the weedy-type melon “Dua dai”. Among these, Dua le, Dua vang, Dua bo, and Dua gang-andromonoecious are cultivated on plains and they formed cluster II along with the reference accessions of Conomon and Makuwa. Based on genetic distance, Dua le and Dua vang were regarded as Makuwa and Dua bo and Dua gang-andromonoecious as Conomon. In contrast, Dua thom and Montok are cultivated in highlands, and they formed cluster III along with landraces from the southern and eastern foot of the Himalayas. Dua gang-monoecious which is commonly cultivated in the southern parts of Vietnam, exhibited the greatest genetic diversity, as explained by its possible origin through the hybridization between Dua gang-andromonoecious and Montok. Genetic differences in melon landraces between plains and highlands and hybridization between these two geographical groups have contributed to the enhancement of genetic diversity in Vietnamese melon.
We found a proportional relationship between the adsorption energy with the gravimetric uptake and the isosteric heat of CO2 adsorption with the volumetric uptake.
–CH3 and –NH2 groups are the best substitutions for improving the activity of the oxygen reduction reaction. The nature of the interaction between the ORR intermediates and porphyrin substrates is charge exchange.
Random amplified polymorphic DNA (RAPD) has been used widely in diversity studies, including population structure and phylogenetics at all taxonomic levels. However, there is a problem in stability and repeatability of RAPD in some cases. Therefore, conversion of RAPD markers into new type of PCR-based marker to overcome low levels of repeatability of RAPD marker is needed. The aim of this study was to develop sequence-tagged site (STS) markers by designing specific primers based on RAPD marker sequences to provide the potential markers for analyzing genetic diversity of melon germplasm. Eight RAPD-STS markers were successfully converted from RAPD markers and have two polymorphism types: A20 and B99 showed different sizes of fragment; A22, A31, A57, B15, B71 and C00 showed presence/absence polymorphism in melon germsplasm. The applicability of new RAPD-STS markers has been demonstrated by comparing genotype analysis of 41 melon accessions using RAPD and RAPD-STS markers. Both of RAPD markers and RAPD-STS markers divided them into two major clusters. However, the RAPD-STS markers were more polymorphic than RAPD markers (polymorphic index content (PIC) values were 0.346 and 0.274, respectively). Mantel's test showed significant correlation (r = 0.896, P < 0.01) between RAPD-STS dendrogram and RAPD dendrogram. Furthermore, RAPD-STS markers could give more information in population structure and identify admixture individuals by using STRUCTURE software. Eight RAPD-STS markers developed in this study are useful for genetic diversity analysis and population studies in melon.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.