Rice (Oryza sativa) allelic sugary1 (sug1) mutants defective in isoamylase 1 (ISA1) accumulate varying levels of starch and phytoglycogen in their endosperm, and the activity of a pullulanase-type of a debranching enzyme (PUL) was found to correlate closely with the severity of the sug1 phenotype. Thus, three PUL-deficient mutants were generated to investigate the function of PUL in starch biosynthesis. The reduction of PUL activity had no pleiotropic effects on the other enzymes involved in starch biosynthesis. The short chains (DP ≤13) of amylopectin in PUL mutants were increased compared with that of the wild type, but the extent of the changes was much smaller than that of sug1 mutants. The α-glucan composition [amylose, amylopectin, water-soluble polysaccharide (WSP)] and the structure of the starch components (amylose and amylopectin) of the PUL mutants were essentially the same, although the average chain length of the B2-3 chains of amylopectin in the PUL mutant was ∼3 residues longer than that of the wild type. The double mutants between the PUL-null and mild sug1 mutants still retained starch in the outer layer of endosperm tissue, while the amounts of WSP and short chains (DP ≤7) of amylopectin were higher than those of the sug1 mutant; this indicates that the PUL function partially overlaps with that of ISA1 and its deficiency has a much smaller effect on the synthesis of amylopectin than ISA1 deficiency and the variation of the sug1 phenotype is not significantly dependent on the PUL activities.
The relationship between the solubility, crystallinity, and length of the unit chains of
plant storage α-glucan was investigated by manipulating the chain length of
α-glucans accumulated in a rice mutant. Transgenic lines were produced by
introducing a cDNA for starch synthase IIa (SSIIa) from an indica
cultivar (SSIIa
I, coding for active SSIIa) into an isoamylase1 (ISA1)-deficient mutant
(isa1) that was derived from a japonica cultivar
(bearing inactive SSIIa proteins). The water-soluble fraction accounted for >95% of the
total α-glucan in the isa1 mutant, whereas it was only
35–70% in the transgenic SSIIa
I
/isa1 lines. Thus, the α-glucans from the SSIIa
I
/isa1 lines were fractionated into soluble and insoluble fractions prior
to the following characterizations. X-ray diffraction analysis revealed a weak B-type
crystallinity for the α-glucans of the insoluble fraction, while no crystallinity
was confirmed for α-glucans in isa1. Concerning the degree of
polymerization (DP) ≤30, the chain lengths of these α-glucans differed
significantly in the order of SSIIa
I
/isa1 insoluble > SSIIa
I
/isa1 soluble > α-glucans in isa1. The amount
of long chains with DP ≥33 was higher in the insoluble fraction α-glucans
than in the other two α-glucans. No difference was observed in the chain length
distributions of the β-amylase limit dextrins among these α-glucans. These
results suggest that in the SSIIa
I
/isa1 transgenic lines, the unit chains of α-glucans were
elongated by SSIIaI, whereas the expression of SSIIaI did not affect
the branch positions. Thus, the observed insolubility and crystallinity of the insoluble
fraction can be attributed to the elongated length of the outer chains due to
SSIIaI.
Amylose and amylopectin of rice mutants deficient in a starch synthase (SS) isozyme in the endosperm, either SSI (ΔSSI) or SSIIIa (ΔSSIIIa), were structurally altered from those of their parent (cv. Nipponbare, Np). The amylose content was higher in the mutants (Np, 15.5%; ΔSSI, 18.2%; ΔSSIIIa, 23.6%), and the molar ratio of branched amylose and its side chains was increased. The chain-length distribution of the β-amylase limit dextrins of amylopectin showed regularity, which appeared consistent with the generally accepted cluster structure, and the degrees of polymerization found at the intersections were taken as the boundaries of the B-chain fractions. The mole % of the B(1)-B(3) fractions was changed slightly in ΔSSI, which is consistent with the proposed role of SSI in elongating the external part of clusters. In ΔSSIIIa, a significant increase in the B(1) fraction and a decrease in the B(2) and B(3) fractions were observed. The internal chain length of the B(2) and B(3) fractions appeared to be slightly altered, suggesting that the deficiency in SS affected the actions of branching enzyme(s).
In this study, we elucidated the plasma pharmacokinetics of MP and its metabolites in male rats after an oral administration. MPOL is most likely to be produced by 11β-HSD1 in the male rats and humans.
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