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ABSTRACT. Lipid and lipoprotein concentrations and apolipoprotein profile were investigated in canine pancreatitis induced by infusion with oleic acid (OA) into the accessory pancreatic duct. Pancreatitis was diagnosed by physical, hematological, biochemical and pathological examinations. In OA-treated dogs, serum triglyceride (TG) concentration was increased; however, there were no changes in serum total cholesterol (TC) and phospholipid (PL) concentrations. Serum concentrations of TG, TC, PL and total lipids (TL) in beta lipoprotein and those of TC, PL and TL in pre-beta lipoprotein were increased and those of TC, PL and TL in alpha 1 lipoprotein were decreased. In apolipoprotein profile, the proportions of apolipoprotein B100 in low density lipoprotein fraction and apolipoprotein A-IV in high density lipoprotein fraction were increased. In addition, decreased proportion of apolipoprotein A-I and the appearance of serum amyloid A protein in high density lipoprotein fraction were observed. These results suggest that lipoprotein profiles observed in canine acute pancreatitis are attributed to the alterations in apolipoprotein compositions. -KEY WORDS: apolipoprotein, canine, lipid, lipoprotein, pancreatitis.J. Vet. Med. Sci. 60(4): 413-421, 1998 in clinical pancreatitis [15,27], increase of TG are not observed in these experimentally induced pancreatitis [4,27]. Therefore, hyperlipidemia in acute pancreatitis might not be reproduced by ligation of pancreatic duct and infusion with mixture of bile and trypsin. In this study, induction of canine acute pancreatitis was attempted by the infusion with oleic acid (OA) into pancreatic duct. Hyperlipidemia is brought by changes of lipoprotein metabolism. Apolipoproteins are structural proteins and participate in lipoprotein metabolism. Changes of apolipoprotein composition bring about abnormality in lipoprotein metabolism. Analysis of apolipoprotein is important to understand the changes of lipoprotein metabolism; however, there is no report on serum apolipoprotein profile in canine pancreatitis. The purpose of this study is to examine the effects of acute pancreatitis induced with OA on serum lipid and lipoprotein concentrations and apolipoprotein profile in dogs. MATERIALS AND METHODSExperimental animals: Eight male beagle dogs, weighing 10.5 to 14.5 kg and aged over one year were used. These dogs were divided into two groups. OA-treated dogs were injected with OA (Sigma Chemical Co., U.S.A.) and control dogs were injected with physiological saline (PS; Otsuka Seiyaku Co., Ltd., Tokyo).Surgical manipulation and inducing pancreatitis: Operative procedures were performed on the dogs after premedication with atropine sulfate (Tanabe Seiyaku Co., Ltd., Osaka) and xylazine hydrochloride (Bayer Japan Co., Ltd., Tokyo) and induction with sodium thiamylal (Yoshitomi Seiyaku Co., Ltd., Osaka) and under inhalation anesthesia with isoflurane (Dainabot Co., Ltd., Tokyo). Laparotomy through a midline approach and duodenotomy was done. A flexible 22-gauge needle was inserted via...