A new basic peptide antibiotic designated as K-582 was isolated, purified and characterized. When K-582 was applied to a column of A1203 or Bio-Gel P-2 or CM Sephadex, two major peaks which were named Fraction I (K-582 A) and Fraction II (K-582 B) were obtained. The nitrogen content, the behavior in color reaction, the absorption bands of amide linkages in the infrared absorption spectrum, 1H NMR spectrum and C-13 NMR spectrum indicated the peptide nature of K-582 A and K-582 B. K-582 was effective against yeasts, but inactive against other Gram-positive bacteria, Gram-negative bacteria and Mycobacterium. The toxicity was low in mice.During our screening program for substances with antibiotic activity, we found a new substance named K-582 produced by the strain 582M which was isolated from a soil sample collected in Sendai and from the results of detailed taxonomical studies the strain was identified as Metarhizium anisopliae (METSCH.) SOROK. Var. anisopliae.The antibiotic, K-582, exhibited a significant growth inhibition of Candida in liquid media, of viruses in tissue culture and of ascites tumor in mice.This paper deals with the characterization of Metarhizium anisopliae (METSCH.) SOROK. var. anisopliae 582 M, the fermentation process, the isolation procedure and the properties of K-582. Taxonomical StudiesStrain 582 M was isolated by SHIGEJI KONDO from a soil sample collected in the vicinity of Sendai City, Miyagi Prefecture, Japan. The taxonomical characterization was carried out according to the methods described by BARNETT1), BARRON2) and TULLOCH3) and the strain 582 M was classified as Metarhizium anisopliae (METSCH.) SOROK. var. anisopliae. Macroscopic descriptionColonies on CZAPEK-peptone agar grew rapidly, attaining a diameter of 4.5 -5.0 cm in 2 weeks at 28°C. The colonies are more or less fasciculated, plane or sometimes lightly furrowed radially. They have white margins with olivaceous to dark olivaceous colored sporulating areas in center. Colonies are often intermixed or zonate. Transformation to light yellow to dark olivaceous colors occurs. Exudate is lacking. The odor was conspicuously pungent similar to that of streptomyces (Tables 1, 2 and 3.).
A derivative of thioscmicarbazone, ƒÁ-thiochromanone-l-thiosemicarbazone (SN-13), which dif-
It is difficult to study subacute thyroiditis epidemiologically since it occurs sporadically and infrequently. Information about 1,127 cases (108 males, 1,019 females, from 1967 to 1982) of subacute thyroiditis in northern Japan was obtained through a questionnaire. It was found that the usual age for the disease was forty, that females predominated in a ratio of 10.6:1, and that the prevalent month was July. In clinical features, the frequencies of the inflammatory symptoms were high in the acute phase of the disease, and the frequencies of hyperthyroid symptoms increased with the progress of the disease. According to the course of the disease (days after the onset without treatment), the patients were divided into seven subgroups, such as 1 approximately 7 days, 8 approximately 14 days, 15 approximately 21 days, 22 approximately 28 days, 29 approximately 42 days, 43 approximately 56 days and over 57 days, respectively. Compared with the 1 approximately 7 days group, the erythrocyte sedimentation rate, serum T4 and T3 concentrations in the 15 approximately 21 days group showed a significant increase from 64 +/- 35 to 75 +/- 30 mm/h (p less than 0.001), 14.6 +/- 5.5 to 17.6 +/- 5.6 micrograms/100 ml (p less than 0.001) and 218 +/- 124 to 263 +/- 109 ng/100 ml (p less than 0.05), respectively, but the BMR showed as insignificant increase from 20 +/- 15 to 24 +/- 14%. The 24-hr 131I-thyroid uptake and resin sponge uptake (RSU) in the 21 approximately 28 days group were 1.2 +/- 1.5% and 35.1 +/- 6.7%, respectively; the former was significantly lower (p less than 0.02) and the latter was insignificantly higher than the values (2.0 +/- 2.6% and 33.9 +/- 7.9%, respectively) in the 1 approximately 7 days group. The recovery time in the steroid-treated group was 57.2 +/- 47.6 days, which showed a statistically insignificant difference from 64.8 +/- 50.5 days of the sodium salicylate-treated group. But the recovery time of 78.2 +/- 64.9 days in other anti-inflammatory drug-treated groups was significantly longer than that of the steroid and sodium salicylate-treated groups (p less than 0.001 and p less than 0.05). Among 9 viral diseases, such as measles, varicella, erythema infection, hand-foot and mouth disease, rubella, mumps, influenza, epidemic keratoconjunctivitis and acute hemorrhagic conjunctivitis observed in northern Japan and Miyagi prefecture in the past 4 years, mumps, hand-foot and mouth disease and epidemic keratoconjunctivitis were prevalent in summer.(ABSTRACT TRUNCATED AT 400 WORDS)
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