Mucosal administration of experimental autoimmune encephalomyelitis (EAE)-specific autoantigens can reduce the onset of disease. To examine whether cholera toxin-B-subunit (CTB)-conjugated EAE-specific T-cell epitope can reduce development of the autoimmune disease in mice, we produced a recombinant hybrid molecule of CTB fusion protein linked with proteolipid-protein (PLP)-peptide139-151(C140S) at levels up to 0.1 gram per liter culture media in Bacillus brevis as a secretion-expression system. Amino acid sequencing and GM1-receptor binding assay showed that this expression system produced a uniformed recombinant hybrid protein. EAE was induced in SJL/J mice by systemic administration with the PLP-peptide. When nasally immunized 5 times with 70 microg rCTB PLP-peptide hybrid protein, mice showed a significantly suppressed development of ongoing EAE and an inhibition of both the PLP-peptide-specific delayed-type hypersensitivity (DTH) responses and leukocyte infiltration into the spinal cord. In contrast, all mice given the PLP-peptide alone or the PLP-peptide with the free form of CTB did not suppress the development of EAE and DTH responses. These results suggest that nasal treatment with the recombinant B. brevis-derived hybrid protein of CTB and autoantigen peptide could prove useful in the control of multiple sclerosis.
: The emergence of lowest‐low fertility in southern and eastern Europe dramatically changed the relationship between fertility and its covariates. Today, low fertility countries are characterized by low female labor force participation, low gender equity, robust marriage institution, and strong familism. It is assumed that the discrepancy between an advanced socioeconomic system and robust family patterns is an important cause of lowest‐low fertility. The Republic of Korea is a typical case where the conventional family pattern causes disharmony with socioeconomic development and depresses fertility. The direct cost of children has been rising rapidly because of the Confucian value of education and strong family ties. The tight labor market conditions and the increasing uncertainty about the future are depressing nuptiality as well as marital fertility. The robustness of traditional gender role division checks the improvement in compatibility between work and the family and sustains the high opportunity cost of childbearing. Although the Korean government announced an ambitious pronatal program in 2006, the budget is so limited that its effect is questionable. Furthermore, a large part of the difference between moderately low and lowest‐low fertility is attributed to cultural patterns and is beyond family policy.
In situ polymerization by certain transition metal catalysts supported on and activated by acid-treated montmorillonite produces well-dispersed clay-polyolefin nanocomposites, without requiring either organic surfactants to be present in the clay phase or modification of the polyolefin structure.
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