In most mammalian species parent-offspring interactions during early life periods primarily comprise social contacts with the mother, whereas the role of males in parental care is one of the most overlooked and understudied topics. The present study addressed the hypothesis that the complete deprivation of paternal care delays or permanently retards synaptic connectivity in the brain, particularly in the medial prefrontal cortex (mPFC) of the offspring in a sex-specific manner. Another aim of this study was to address the question whether and in which way replacing the father with a female caregiver (in our experiments the "aunt") can "buffer" the detrimental effects of paternal deprivation on neuronal development. The comparison of: (a) single mother rearing; (b) biparental rearing by father and mother; and (c) biparental rearing by two female caregivers revealed that: (i) paternal care represents a critical environmental factor for synaptic and dendritic development of pyramidal neurons in the vmPFC of their offspring; (ii) a second female caregiver ("aunt") does not "buffer" the neuronal consequences of paternal deprivation; and that (iii) neuronal development in the vmPFC is differentially affected in male and female offspring in response to different family constellations.
While the majority of studies on the importance of parental caregiving on offspring behavioral and brain development focus on the role of the mother, the paternal contribution is still an understudied topic. We investigated if growing up without paternal care affects dendritic and synaptic development in the nucleus accumbens of male and female offspring, and if replacement of the father by a female caregiver “compensates” the impact of paternal deprivation. We compared a) biparental rearing by father and mother b) mono-parental care by a single mother and c) biparental rearing by two female caregivers. Quantitative analysis of medium-sized neurons in the nucleus accumbens revealed that growing up without father resulted in reduced spine number in both male and female offspring in the core region, whereas spine frequency was only reduced in females. In the shell region reduced spine frequency was only found in males growing up in a monoparental environment. Replacement of the father by a female caregiver did not “protect” against the effects of paternal deprivation, indicating a critical impact of paternal care behavior on the development and maturation of neuronal networks in the nucleus accumbens.
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