Dilated cardiomyopathy (DCM) is characterized by the left ventricular dilatation and impaired myocardial systolic dysfunction with high mortality and morbidity. However, the underlying mechanisms remain elusive. We first identified the differentially expressed genes (DEGs) between the DCM and control group using two expression profiles from GSE3585 and GSE84796. Enrichment analysis was conducted to explore the potential mechanisms underlying DCM. A total of four algorithms, including key module of MCODE, degree, maximum neighborhood component (MNC), and maximal clique centrality (MCC), were used to identify the hub genes within Cytoscape. The correlation between hub genes and infiltrated immune cells was evaluated to determine potential immune-related genes. The expression analysis and diagnosis value analysis of potential immune-related genes were performed. Finally, the expression analysis with GSE57338 and relationship analysis with the comparative toxicogenomics database (CTD) were performed to identify the key immune-related genes in DCM. A total of 80 DEGs were screened for DCM. Enrichment analysis revealed that DEGs were involved in the immune-related pathological process. Immune infiltration analysis indicated a potentially abnormal immune response in DCM. Four up-regulated genes (COL1A2, COL3A1, CD53, and POSTN) were identified as potential immune-related genes. Finally, three genes (COL1A2, COL3A1, and POSTN) were determined as the key immune-related genes in DCM via expression analysis with a validation set (GSE57338) and relationship analysis with CTD. Our study suggested that the upregulated COL1A2, COL3A1, and POSTN might be the key immune-related genes for DCM. Further studies are needed to validate the underlying mechanisms.
BackgroundAccumulating researches have indicated that cirrhosis is a vital risk factor for morbidity and mortality worldwide. Nevertheless, the underlying immune-related molecular mechanism remains indistinct.MethodsGene expression profiles of GSE89377 and GSE139602 were investigated to identify differentially expressed genes (DEGs) related to cirrhosis. Enrichment analysis for DEGs was explored. CIBERSORT algorithm was used for evaluating DEGs immune infiltration. The String and Cytoscape database were utilized for analyses hub DEGs with a high tight connection, and the association between hub DEGs and immune cells infiltration was analyzed by Spearman method. Finally, the underlying molecular mechanism of the key DEGs was predicted via KEGG pathway analysis.ResultsIn all, 299 DEGs were attained among them 136 and 163 were up and down-regulated respectively. Then Enrichment function of DEGs and CIBERSORT algorithm showed that they are significant in immune and inflammatory responses. Four hub DEGs (ACTB, TAGLN, VIM, SOX9) were identified. Subsequently, the immune infiltration findings indicated that, the hub DEGs highly related immune cells. Finally, KEGGs pathways were predicted related with ACTB. ConclusionsThis study revealed key DEGs may implement inflammatory immune responses with cirrhosis, which could be used as biomarkers or therapeutic targets.
Background:Dilated cardiomyopathy (DCM) is characterized by the left ventricular dilatation and impaired myocardial systolic dysfunction with high mortality and morbidity. However, the underlying mechanisms remain elusive.Methods:Differentially expressed genes (DEGs) analysis was performed using two expression profiles from GSE3585 and GSE8479. Enrichment analysis to explore the potential mechanisms underlying DCM was conducted. A total of four algorithms were used within Cytoscape to identify the hub genes. Correlation analysis between hub genes and infiltrated immune cells to identify potential immune-related genes. The expression analysis and diagnosis value analysis of potential immune-related genes were performed. Finally, the expression analysis with GSE57338 and relationship analysis with the comparative toxicogenomics database (CTD) were performed for identification of the key immune-related genes in DCM. Results:A total of 80 DEGs were screened for DCM. Enrichment analysis revealed that DEGs were involved in immune-related pathological process. Immune infiltration analysis indicated a potential immune response abnormal in DCM. Four up-regulated genes, including COL1A2, COL3A1, CD53, and POSTN, were identified as potential immune-related genes. Finally, three genes (COL1A2, COL3A1, and POSTN) were determined as the key immune-related genes in DCM via expression analysis with a validation set (GSE57338) and relationship analysis with CTD.Conclusions:Our study suggested that the upregulated COL1A2, COL3A1, and POSTN might be the key immune-related genes for DCM. Further studies are needed to validate the underlying mechanisms.
Background: Dilated cardiomyopathy (DCM) is characterized by the left ventricular dilatation and impaired myocardial systolic dysfunction with high mortality and morbidity. However, the underlying mechanisms remain elusive. Methods: Differentially expressed genes (DEGs) analysis was performed using two expression profiles from GSE3585 and GSE84796. Enrichment analysis to explore the potential mechanisms underlying DCM was conducted. A total of four algorithms, including key module of MCODE, degree, maximum neighborhood component (MNC), and maximal clique centrality (MCC), were used within Cytoscape to identify the hub genes. Correlation analysis between hub genes and infiltrated immune cells to identify potential immune-related genes. The expression analysis and diagnosis value analysis of potential immune-related genes were performed. Finally, the expression analysis with GSE57338 and relationship analysis with the comparative toxicogenomics database (CTD) were performed for identification of the key immune-related genes in DCM. Results: A total of 80 DEGs were screened for DCM. Enrichment analysisrevealed that DEGs were involved in immune-related pathological process. Immune infiltration analysis indicated a potential immune response abnormal in DCM. Four up-regulated genes, including COL1A2, COL3A1, CD53, and POSTN, were identified as potential immune-related genes. Finally, three genes (COL1A2, COL3A1, and POSTN) were determined as the key immune-related genes in DCM via expression analysis with a validation set (GSE57338) and relationship analysis with CTD. Conclusions: Our study suggested that the upregulated COL1A2, COL3A1, and POSTN might be the key immune-related genes for DCM. Further studies are needed to validate the underlying mechanisms.
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