Acetate overflow refers to the metabolism by which a large part of carbon incorporated as glucose into Escherichia coli cells is catabolized and excreted as acetate into the medium. We previously found that mutants for the acetate overflow pathway enzymes phosphoacetyltransferase (Pta) and acetate kinase (AckA) showed significant diauxic growth after glucose depletion in E. coli. Here, we analyzed the underlying mechanism in the pta mutant. Proteomic and other analyses revealed an increase in pyruvate dehydrogenase complex subunits and a decrease in glyoxylate shunt enzymes, which resulted from pyruvate accumulation. Since restoration of these enzyme levels by overexpressing PdhR (pyruvate‐sensing transcription factor) or deleting iclR (gene encoding a pyruvate‐ and glyoxylate‐sensing transcription factor) alleviated the growth lag of the pta mutant after glucose depletion, these changes were considered as the reason for the phenotype. Given the evidence for decreased coenzyme A (HS‐CoA) levels in the pta mutant, the growth inhibition after glucose depletion was partly explained by limited availability of HS‐CoA in the cell. The findings provide insights into the role of acetate overflow in metabolic regulation, which may be useful for biotechnological applications.
We propose a university-class micro-satellite "Hu-ring " to localize and study gamma-ray bursts. The primary mission of Hu-ring is to localize gamma-ray bursts with an 10 accuracy in real time, and transmit promptly the coordinates to the ground. Although many of its mission concepts are modeled after HETE-2, use of avalanche photodiodes (APDs), innovative photon detector device, make it possible to further reduce the size and the mass of the satellite. We designed Hu-ring within a size of 50 cm cube and a weight limit of 50 kg, so that it can be launched as a piggy-back payload of the Japanese H-IIA Launch Vehicle. The satellite is spin-stabilized, and has a half-sky field of view centered on the anti-sun direction. A set of scintillation counters equipped with rotation modulation collimators are employed for localization of GRBs. We also measure the soft/medium X-ray spectra of GRBs using APDs as a direct X-ray photon detectors. These two kinds of instruments cover the 0.5-200 keV energy range. The satellite bus is designed mostly with commercially available components in order to reduce the cost and the lead time. Following the HETE-2 model, in order to receive the prompt burst alerts it is designed to rely on a global network of receive-only low-cost ground stations, which may be hosted at research instutions with a small footprint. We performed analyses in many aspects: mechanical and thermal design of the satellite bus, attitude control simulations, power budget, ground contact schedule and downlink capacity, etc. We verified that the mission goal can be achieved with this proposed design philosophy.
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