The purpose of this investigation was to evaluate the antimicrobial activity of a novel quaternary ammonium silane coupling agent, N-allyl-N-decyl-N-methyl-Ntrimethoxysilylpropylammonium iodide (10-I), against early stage biofilms using a polymicrobial biofilm model that simulates oral plaque-like biofilm formation on a solid phase. Cover glasses were immersed in a sufficient volume of the 800-ppm solution of 10-I for 1 h to modify the surface. Aliquots of a mixture of stimulated saliva from a single subject and McBain medium were dispensed into polystyrene 24-well cell culture plates and then cultured at 37.0°C under anaerobic condition. After culturing, we calculated colony forming unit (CFU). CFU of the 10-I group was approximately 80% lower than that of the control group, demonstrating strong antimicrobial activity. Therefore, surface modification with 10-I is effective for treating oral indigenous bacteria-related dental diseases found in the elderly and immunocompromised individuals and possible systemic complications, such as aspiration pneumonia.
Background: Silane coupling agents are used as a bonding agent between ceramics and resins in dentistry. The effectiveness of these agents is often enhanced by the addition of acid or by heat treatment. Objective: This study aimed to evaluate, the clinical characteristics, TBS (tensile bond strength) and water resistance of eight commercial ceramic primers (seven silane coupling agents and one alumina zirconia primer). Method: Glass plates were used as the adherent. The TBS of the resin composite and the wettability of the resin monomer to glass surfaces treated with ceramic primers was investigated, with 3-MPS (methacryloxypropyltrimethoxysilane) used as a control. The values obtained from the experiments were analyzed using one-way ANOVA followed by Tukey's multiple comparison tests (p < 0.05). Results: No significant differences in TBS were observed between the commercial products and MPS, except for the alumina zirconia primer. However, four products (three of the seven agents and the alumina zirconia primer) exhibited significantly lower TBS values after application of thermal stress when compared with those stored in water (p < 0.05). All but one of the eight primers displayed significantly higher contact angles between the treated glass and the resin monomer when compared with MPS (p < 0.05). These results suggest that some commercial ceramic primers may contribute to increased strength and durability.
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