The aim of our study was to evaluate the parasite -host interactions at apoptosis level. We studied histopathological changes and time course of apoptosis in the duodenum during Eimeria acervulina infection. One-day-old broiler chicks were randomly allocated into two equal groups. At the age of two weeks the first group was experimentally infected with a pure suspension of sporulated E. acervulina oocysts. The second group served as a negative control. Tissue samples from the upper part of duodenum were obtained at 0.5, 1, 2, 3, 4, 5 and 6 days post infection. Biopsies of duodenum were studied immunohistochemically using DeadEnd TM Colometric TUNEL System for apoptosis detection in duodenal mucosa. Number of parasites in duodenal epithelium was also investigated. Our experimental results demonstrate: (i) macroscopic and histopathological changes in epithelium detected mainly in proximal segment of duodenum in infected groups; (ii) the number of developmental stages of E. acervulina (DSEA) during our trial increased, reaching the maximum 5 days post infection (dpi) (332.2 ± 16.12) (mean ± SEM), whereas the amount of DSEA declined significantly as late as 6 dpi (124.6 ± 3.91); (iii) the highest apoptosis level was recorded in initiatory 0.5 dpi (13.2 ± 1.02) and on the end of parasite development cycle after 5 dpi (12.6 ± 1.36). Finally, results showed that there was a period of inhibition of apoptosis during infection by E. acervulina.
Obesity and overweight have been frequently observed in dogs in recent years as in humans. The compositions of fatty acids in the accumulated lipids in tissues of obese animals may have important roles in the process and mechanisms related to the onset of metabolic disorders. The purpose of this study was to evaluate the effects of a vegetarian diet, which contained a higher proportion of unsaturated fatty acids on individual morphometric parameters in spayed dogs. Seven mongrel dogs without ideal body condition scores (average: 7.42) were fed vegetarian dog kibbles and received the daily amount of energy calculated with the following formula: 130 kcal × weight (kg) 0.75. The animals were evaluated on days 0 and 60 in relation to the body measurements, such as: body weight, metabolic body weight, body fat percentage, nape, rib, tail base, chest, and abdomen, using a plicometer. Also determined were the body condition scores (scale from 1—thin to 9—obese), canine body mass index, and their waist circumference. These data were analysed by the Student’s t-test. The body measurement variation of body fat percentage, waist circumference, body condition score and thickness values of the abdomen differed significantly (P < 0.01).
If gastric acidity is to be encouraged, it may be beneficial to eliminate some feed elements from pig starter meals since they bind more acid in the stomach than others. The cereals (wheat, maize, and barley), soybean meal, commercial mineral supplement, and two feed mixtures (one of them with the additive Zeolit) were evaluated. In this study, it was discovered that adding zeolite to the feed mixture had the effect of lowering its initial pH (P<0.001), buffering (P<0.05) and binding capacity (P<0.05). This study aimed to measure the buffering and acid-binding capacity of some ingredients commonly used in growing pig feeds and estimate the coefficient of correlation between crude protein, ash, and the buffering capacity of feed ingredients with significant linear correlation (P<0.05 and P<0.01, respectively). The combined impact of the individual feed mixture components' individual buffering capacities has not been proven.
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