A method to visualize in situ of single strand scissions of DNA in fixed mammalian cells has been developed. Using the nuclear nick translation with biotin-labeled dUTP followed by binding to avidin-biotin-peroxydase complex, the nuclei of HeLa cells which had been treated with a DNA-damaging antibiotic bleomycin were specifically stained, implicating that the histochemical detection of single strand scissions (nicks) of DNA in fixed cells was completed without destroying the morphology, and without using autoradiography.
Lateral pharyngoesophageal diverticulum is a rare disorder. 1-3 Although its radiologic appearance is well known, to our knowledge there has been no case reports of a lateral pharyngoesophageal diverticulum identified by ultrasound.We report a case in which a lateral pharyngoesophageal diverticulum was filled with dietary residue and thus simulated a thyroid adenoma on sonography .
CASE REPORTA 66-year-old woman was referred to our hospital with the chief complaints of hand tremor and palpitation. Laboratory test revealed increased triiodothyronine (T3) and thyroxine (T4) together with decreased thyroid stimulating hormone (TSH). On physical examination, in addition to the diffuse enlargement of the thyroid, a nodule was palpated in the left lobe of the thyroid. Ultrasonography was performed using a real-time linear array unit with 5 MHz transducer. It demonstrated an echogenic mass surrounded by a sonolucent band in the posterior portion of the left thyroid lobe (Fig. 1). 99mTc-pertechnetate thyroid scan also revealed a decreased uptake area in the midportion of the left thyroid lobe (Fig. 2). A provisional diagnosis of Graves' disease with a thyroid adenoma was made at that time. The patient was treated with antithyroid drugs and an operation for the adenoma was contemplated.The next week, a second ultrasonography was
Observations on the spleen of chickens aging one day to 8 weeks showed that the development of the lymphoid tissue around Schweigger-Seidel ellipsoids proceeded quite independently of that of the thymus-dependent periarterial lymphoid tissue. These periellipsoidal lymphoid cells were considered to be bursa dependent because of their depletion in bursaless chickens and their consistent increase irrespective of extirpation or destruction of the thymus. bursa of Fabricius; lymphoid tissue; spleen
An epithelial cell line, which is capable of inducing the differentiation of rat bone marrow cells into T cell lineage, has been established from a normal rat thymus. Thymus tissues were minced and dispersed with collagenase and trypsin. About 8 months after the initiation of cultures, the IT-45Rl line was established by utilizing the difference in the strength of adhesiveness of epithelial cells and fibroblasts. IT-45R1 line cells were identified as epithelial cells of thymic origin because of their mosaic-like a.rrangement, desmosomes and tonofilaments, as previously described with regard to IT-26R2l cells (12). The generation doubling time of this line was calculated to be 60 hr. Karyotype analysis showed that the chromosome number ranged from 57 to 76 with the mode of 67.Supernatant from this line was collected in a serum-free medium and examined for its ability to induce the differentiation of T lymphocytes. Rat bone marrow cells were fractionated by discontinous density gradient of bovine serum albumin and incubated with the supernatant. After incubation, the cells were subjected to the cytotoxic test and the assay for rosette formation. Thyl-I surface antigen in fraction 5 cells and rosette forming cells in fraction 4 cells were significantly increased.These results suggest that thymic epithelial cells of IT-45Rl line induce the differentiation of bone marrow cells into immature T lymphocytes.
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