A strain of Rhizobium isolated from the root nodule of lentil was caused to produce mutants and screened on a medium of pH 4-5. Five mutant colonies of Rhizobium were isolated from lentil which differed in growth over a pH range 4-5-7-5. Strains differed in their growth in acidic and calcareous soils and also in their effectiveness of N fixation.
Pigeonpea is one of the most popular legume grains in the world, especially in the Indian sub-continent. Keeping in view the importance of the crop, an efficient protocol for rapid in vitro plant regeneration from cotyledon of redgram has been reported in this paper. The concentraion of the hormones played an important role in shoot and root regeneration.
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