Ecdysteroids mediate a wide variety of developmental and physiological events in insects. In the postembryonic development of insects, ecdysone is synthesized in the prothoracic gland (PG). Although many studies have revealed the biochemical and physiological properties of the enzymes for ecdysteroid biosynthesis, most of the molecular identities of these enzymes have not been elucidated. Here we describe an uncharacterized cytochrome P450 gene, designated Cyp306a1, that is essential for ecdysteroid biosynthesis in the PGs of the silkworm Bombyx mori and fruit fly Drosophila melanogaster. Using the microarray technique for analyzing gene expression profiles in PG cells during Bombyx development, we identified two PG-specific P450 genes whose temporal expression patterns are correlated with changes in ecdysteroid titer during development. Amino acid sequence analysis showed that one of the Bombyx P450 genes belongs to the CYP306A1 subfamily. The temporal and spatial expression pattern of the Drosophila Cyp306a1 homolog is essentially the same as that of Bombyx Cyp306a1. We also found that Drosophila Cyp306a1 is disrupted in the phantom (phm) mutant, known also as the Halloween mutant. The morphological defects and decreased expression of ecdysone-inducible genes in phm suggest that this mutant cannot produce a high titer of ecdysone. Finally we demonstrate that S2 cells transfected with Cyp306a1 convert ketodiol to ketotriol via carbon 25 hydroxylation. These results strongly suggest that CYP306A1 functions as a carbon 25 hydroxylase and has an essential role in ecdysteroid biosynthesis during insect development.

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Under favorable circumstances the time‐dependent aseismic deformation resulting from the loading of the lithosphere by the stress drop of large dip slip earthquakes can be used to determine both the effective elastic plate thickness and the asthenospheric viscosity. The deformation has several similarities with the deflection of the lithosphere by surface loads and with movements due to postglacial rebound. Level changes obtained in the 80 years since the M = 7.5, 1896 Riku‐u earthquake, an intraplate thrust event in northern Honshu, provide convincing evidence that asthenospheric readjustments are responsible for the observed movements. Leveling surveys crossing the zone of surface faulting have been repeated five times since 1900 and delineate a localized depression that has subsided at a continually decreasing rate. The depression is centered close to the 1896 faulting, and its shape and width, about 75 km, are matched by our model using a plate thickness of 30 km. The decaying subsidence rate constrains the viscosity of the uppermost asthenosphere to be 1×1020 P. A linear viscous rheology matches the observed decay quite well, although measurements are sparse during the several decades following the earthquake.

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Assessment of long-term seismic hazard is critically dependent on the behavior of tail of the distribution function of rare strongest earthquakes. Analyses of empirical data cannot however yield the credible solution of this problem because the instrumental catalogs of earthquake are available only for a rather short time intervals, and the uncertainty in estimations of magnitude of paleoearthquakes is high. From the available data, it was possible only to propose a number of alternative models characterizing the distribution of rare strongest earthquakes. There are the following models: the model based on the Guttenberg -Richter law suggested to be valid until a maximum possible seismic event (М mах ), models of 'bend down' of earthquake recurrence curve, and the characteristic earthquakes model. We discuss these models from the general physical concepts supported by the theory of extreme values (with reference to the generalized extreme value (GEV) distribution and the generalized Pareto distribution (GPD) and the multiplicative cascade model of seismic regime. In terms of the multiplicative cascade model, seismic regime is treated as a large number of episodes of avalanche-type relaxation of metastable states which take place in a set of metastable sub-systems.The model of magnitude-unlimited continuation of the Guttenberg -Richter law is invalid from the physical point of view because it corresponds to an infinite mean value of seismic energy and infinite capacity of the process generating seismicity. A model of an abrupt cut of this law by a maximum possible event, М mах is not fully logical either.A model with the 'bend-down' of earthquake recurrence curve can ensure both continuity of the distribution law and finiteness of seismic energy value. Results of studies with the use of the theory of extreme values provide a convincing support to the model of 'bend-down' of earthquakes' recurrence curve. Moreover they testify also that the 'bend-down' is described by the finite distribution law, i.e. the bend-down occurs more efficiently than it is envisaged in the commonly used model developed by Y. Kagan (which treats the bend-dawn as an exponential decay law). However, despite the finiteness of the distribution law, density of magnitudes decline quite slowly in the area close to the maximum possible М mах event as (М mах -M) n , where n varies in the range between 4 and 6 in the majority of cases. As a result М mах value can be estimated only with a large error. In rare cases, if the space-and-time area under study contains higher number of strongest earthquakes, the empirical distribution law becomes close to the exponential law; in this case n value is quite high, and М mах values becomes unstable and tend to infinite growth.In our study, the distribution law of strongest earthquakes was investigated by the methods based on the extreme values theory (world data and several regional catalogues were examined), and the results of calculation do not reveal cases of occurrence of characteristic events. ...

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