Public transport generally addresses the evident mobility needs and offers an often-irreplaceable service, especially for captive users and other disadvantaged population groups. Public transport design and services are closely related to the physical size of modern cities, the number of people living or working in them, and the distribution and organization of work and social activities. However, public transport has been restricted with the spread of the COVID-19 pandemic in Italy, since March 2020. Public transport demand collapsed, especially during the lockdown period (March-May 2020), and adverse effects were reported even in the subsequent periods. In fact, the social distancing restrictions have highlighted numerous problems with public transport systems worldwide, primarily due to two factors. The first is related to the spread of the virus via the respiratory route, which is more likely to infect in restricted areas, and the second is associated with a transport system that by definition has high occupancy rates and low spacing throughout the journey (e.g., the positioning of seats or standing places in a train or bus). Thus, the COVID-19 pandemic has substantially impacted the travel choices of users. The pandemic has also negatively affected the psychological state, generating specific problems of anxiety, fear, or stress among all population groups, even when choosing the means of transport to travel with.Given the emerging pandemic challenges, the present study examines the public transport demand characteristics during the various phases of the COVID-19 pandemic in Sicily, one of the most affected regions in Italy. The study investigates the mental state of a population sample that frequently used the local urban or regional public transport to travel to work before and during the pandemic phases in the Sicilian territory. Through the administration of an online survey, it was possible to collect sociodemographic and psychological data to understand the propensity to use public transport. A series of inferential statistical tests were applied to assess the correlation of psychological aspects (i.e., fear, anxiety, and stress) with socio-demographic variables and modal choice habits (trip frequency). Results highlight and evaluate each psychological issue among population groups and their relative role in shaping public transport-related preferences. The study highlights some proposals and their implementation strategies to prevent negative emotions and encourage public transport use in Sicily and generally.