Background: Inappropriate use of antimicrobial agents in animal production has led to the development of antimicrobial resistance (AMR) in foodborne pathogens. Transmission of AMR foodborne pathogens from reservoirs, particularly chickens to the human population do occur. Recently, we reported that occupational exposure was a risk factor for multidrug-resistant (MDR) Escherichia coli (E. coli) among poultry-workers. Here we determined the prevalence and genetic relatedness among MDR E. coli isolated from poultry-workers, chickens, and poultry environments in Abuja, Nigeria. This study was conducted to address the gaps identi ed by the Nigerian AMR situation analysis. Methods: We conducted a cross-sectional study among poultry-workers, chickens, and poultry farm/live bird market (LBM) environments. The isolates were tested phenotypically for their antimicrobial susceptibility pro les, genotypically characterized using whole-genome sequencing (WGS) and in silico multilocus sequence types (MLST). We conducted a phylogenetic single nucleotide polymorphism (SNPs) analysis to determine relatedness and clonality among the isolates. Results: A total of 115 (26.8%) out of 429 samples were positive for E. coli. Of these, 110 isolates were viable for phenotypic and genotypic characterization. The selection comprised 47 (42.7%) isolates from poultry-workers, 36 (32.7%) from chickens, and 27 (24.5%) from poultry-farm or LBM environments. Overall, 101 (91.8%) isolates were determined MDR conferring resistance to at least three drug classes. High frequency of resistance were observed for tetracycline (n = 102; 92.7%), trimethoprim/sulfamethoxazole (n = 93; 84.5%), streptomycin (n = 87; 79.1%) and ampicillin (n = 88; 80%). Two plasmid-mediated colistin genes-mcr-1.1 harboured on IncX4 plasmids were detected in environmental isolates. The most prevalent sequence types (ST) were ST-155 (n = 8), ST-48 (n = 8) and ST-10 (n = 6). Two isolates of human and environmental sources with a SNPs difference of 6161 originating from the same farm shared a novel ST. The isolates had similar AMR genes and plasmid replicons. Conclusion: MDR E.coli isolates were prevalent amongst poultry-workers, poultry, and the poultry farm/LBM environment. The emergence of MDR E. coli with novel ST in two isolates may be plasmidmediated. Competent authorities should enforce AMR regulations to ensure prudent use of antimicrobials to limit the risk of transmission along the food chain.
The study investigated the incidence of Listeria monocytogenes in ready‐to‐eat (RTE) vegetables: (Cucumber, Cucumis sativas; Cabbage, Brassica olerecea; Carrot, Daucus carota; Tomato, Solanum lycopersicum; Lettuce, Lactuca sativa) in six states in South‐Western Nigeria. A total of 555 composite samples were collected from 30 traditional markets within the states and only 244 L. monocytogenes species were isolated by standard methods. The incidence of L. monocytogenes was low and not statistically significant per vegetable type. The percentage distribution of the L. monocytogenes isolates in the RTE vegetables was 28.28, 9.02, 23.36, 19.67, and 19.67 for Cabbage, Carrot, Cucumber, Lettuce, and Tomatoes, respectively. Lagos state had the highest incidence of L. monocytogenes contamination (55%) followed by Ondo (48.89%), Oyo (48.75%), Ogun (44.09%), Osun (34.38%), and Ekiti (33.33%) states, respectively. Although the incidence of L. monocytogenes in the RTE vegetables in South‐Western Nigeria is low, its presence should be a source of concern as it could constitute a public health threat for its consumers.
Poly-γ-glutamic acid (γPGA) is a natural and promising biopolymer synthesized by Bacillus spp. during fermentation. This study isolated Bacillus spp. from ogi steep liquor (OSL) and lemon- ogi steep liquor (LOSL) using standard methods and determined the γPGA-producing ability. The antimicrobial and angiotensin-converting enzyme (ACE) inhibitory activities of γPGA polymer were evaluated and isolates were sequenced. Four isolates (TA004, TA006, TA011, TA012) selected based on phenotypic characterization and stickiness (<15 cm) showed antibacterial activity against different pathogens with the highest activity found in TA004 (22.5 mm) and least in TA011 (16.6 mm). Furthermore, time-kill assay showed that the combined γPGA polymer was more effective and demonstrated bactericidal activity over individual γPGA which are bacteriostatic in nature. All γPGA polymer exhibited ACE properties except TA011. The highest IC 50 was observed in TA006 (0.11 mg/ml) and least in TA004 (0.35 mg/ml). TA004 had the highest molecular weight (261 kDa) while TA011 had the least (194.97 kDa). In addition, all γPGA exhibited characteristic peaks at 3413–3268 cm -1 and 1722–1664 cm -1 that corresponded to amine N–H stretching intensities and C = O stretching in COOH. Bacillus isolates were identified as TA004 ( B. subtilis -GenBank: MH782061), TA006 ( B. amyloliquefaciens - GenBank: MH782075), TA011 ( B. subtilis - GenBank: MH782088), TA012 ( B. subtilis - GenBank: MH782083). OSL and LOSL have the potential for developing functional foods with a valuable effect on health.
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