Cancer is one of the most lethal diseases. Recently, cancer immunotherapy has a tremendous achievement in cancer treatment. A certain number of cancer based epitope vaccines with different moiety have been discovered. In japan, several clinical tests of cancer based epitope vaccine derived from tumor associated antigens (TAAs) are now ongoing or have recently been completed. a novel of TAAs potentially as cancer vaccine have been retrieved from a fragment weighed 48kDa derived from human DNA-topoisomerase 1 (TOP1) called Topo48. Therefore, it is still critical to discover a derived Topo48 epitope based cancer vaccine. Immuno-informatics considered as a methods noted to have better accuracy to design promising vaccine candidates. Here, continuous and discontinuous B-cell epitopes following with CTL epitopes and their docking interaction to major histocompatibility complex (MHC) class I Human Leukocyte Antigens (HLA)- A0201 were predicted. Kolaskar-Tongaonkar’s, Emini’s, Karpus-Schulz’s, and Parker’s methods were used to predict continuous B-cell epitopes while ElliPro was used for prediction of discontinued B-cell epitopes. Those considered methods marked to have better accuracy to design promising vaccine candidates. Similarly, CTL epitopes was also predicted by using NetCTL server and the best candidates were further investigated their binding affinity by mean of PEP-FOLD3, PatchDock rigid-body docking server, and FireDock server. Total 27 continuous epitopes and 7 discontinuous B-cell epitopes were predicted. In the other hand, 9 peptides were predicted as CTL epitopes. Whereas, three predicted CTL epitope in range 263MLDHEYTTK27, 755AIDMADEDY763, 715ALGTSKLNY724) exhibited good interactions to HLA-A0201. Moreover, we also found residues His266, Thr270, Ala755, Tyr723, Thr718, Ser719, Lys720 from Topo48 and residues Thr163, Asp757, His70, Glu63 from HLA- A0201 were indicated to be antigenic. Ultimately, our proposed continuous/discontinuous B-cell epitopes, and also CTL epitopes can be potential vaccines for cancer immunotherapy.
Salah satu permasalahan yang muncul pada mitra kegiatan Pengabdian kepada Masyarakat ini adalah belum tersedianya sistem integrasi data kependudukan dari masing-masing pekon/desa ke kecamatan. Kegiatan ini bertujuan untuk melakukan pelatihan, pendampingan serta demonstrasi sistem integrasi yang diusulkan. Solusi yang akan ditawarkan adalah dengan melakukan pelatihan dan sosialisasi terkait dengan pengelolaan dan integrasi data kependudukan. Pelatihan dan sosialisasi ini akan menggunakan alat bantu Ms. Excel serta Google Sheets pada prosesnya. Metode yang digunakan dalam pelaksanaan kegiatan pengabdian kepada masyarakat adalah pelatihan dan pendampingan serta demonstrasi terkait dengan Penerapan Sistem Integrasi Data Kependudukan Sederhana (SIDaKS) di Kecamatan Kota Agung Timur, Tanggamus. Pelaksanaan pelatihan dilaksanakan di aula Kecamatan Kota Agung Timur, Tanggamus dengan dihadiri oleh 43 peserta yang berasal dari beberapa perwakilan setiap pekon/desa di lingkungan Kecamatan Kota Agung Timur. Hasil yang telah dicapai antara lain peningkatan pemahaman peserta yang telah signifikan secara uji statistika serta suatu usulan sistem integrasi data kependudukan.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.