Umbilical cord mesenchymal stem cells (UC-MSCs) have been suggested as a candidate for various clinical applications, however, major limitations include the lack of organ-specific accumulation and low survival rates of transplanted cells. In the present study, it was hypothesized that the paracrine effects of UC-MSCs may enhance stem cell-based tissue repair and regeneration by promoting the specific homing of stem/progenitor cells and the overall ability to drive them to the damaged area. UC-MSCs-derived conditioned medium (UC-CM) was analyzed using liquid chip and ELISA techniques. In vitro tube formation assays of human umbilical vein endothelial cells (HUVECs) and UC-MSCs were then performed to assess the angiogenic properties of UC-CM. Subsequently, UC-MSCs, HUVECs and fibroblasts were labeled with PKH26 for an in vivo cell migration assay. The expression levels of C-X-C chemokine receptor 4 (CXCR4), C-C chemokine receptor 2 (CCR2) and c-met were determined in the UC-MSCs, HUVECs and fibroblasts using reverse transcription-quantitative polymerase chain reaction and flow cytometry. UC-CM was incubated with or without antibodies, and the contribution of stromal cell-derived factor 1 (SDF-1), monocyte chemotactic protein 1 (MCP-1) and hepatocyte growth factor (HGF) on the migration of cells was investigated in vitro. The results demonstrated that UC-MSCs secreted different cytokines and chemokines, including increased quantities of SDF-1, MCP-1 and HGF, in addition to the angiogenic factors, vascular cell adhesion protein-1, interleukin-8, insulin-like growth factor-1 and vascular endothelial growth factor. The total lengths of the tubes were significantly increased in the UC-MSCs and HUVECs incubated in UC-CM compared with those incubated in Dulbecco’s modified Eagle’s medium. In vivo cell migration assays demonstrated that UC-CM was a chemotactic stimulus for the UC-MSCs and HUVECs. In vitro Matrigel migration and scratch healing assays demonstrated that UC-CM increased the migration of CXCR4-postive or/and CCR2-positive cells in a dose-dependent manner. In addition, different molecules were screened under antibody-based blocking migration conditions. The data revealed that the SDF-1/CXCR4 and MCP-1/CCR2 axes were involved in the chemoattractive activity of UC-CM and suggested that the effective paracrine factor of UC-CM is a large complex rather than a single factor. The results of the present study supported the hypothesis that UC-MSCs release soluble factors, which may extend the therapeutic applicability of stem cells.
Topographically induced upwelling caused by the interaction between large-scale currents and topography was observed during four cruises in the northern South China Sea (NSCS) when the upwelling favorable wind retreated. Using a high-resolution version of the Princeton Ocean Model, we investigate relative contributions of local wind and topography to the upwelling intensity in the NSCS. The results show that the topographically induced upwelling is sensitive to alongshore large-scale currents, which have an important contribution to the upwelling intensity. The topographically induced upwelling is comparable with the wind-driven upwelling at surface and has a stronger contribution to the upwelling intensity than the local wind does at bottom in the near-shore shelf region. The widened shelf to the southwest of Shanwei and west of the Taiwan Banks intensifies the bottom friction, especially off Shantou, which is a key factor for topographically induced upwelling in terms of bottom Ekman transport and Ekman pumping. The local upwelling favorable wind enhances the bottom friction as well as net onshore transport along the 50 m isobath, whereas it has less influence along the 30 m isobath. This implies the local wind is more important in upwelling intensity in the offshore region than in the nearshore region. The contribution of local upwelling favorable wind on upwelling intensity is comparable with that of topography along the 50 m isobath. The effects of local upwelling favorable wind on upwelling intensity are twofold: on one hand, the wind transports surface warm water offshore, and as a compensation of mass the bottom current transports cold water onshore; on the other hand, the wind enhances the coastal current, and the bottom friction in turn increases the topographically induced upwelling intensity.
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