Additional supporting information may be found online in the Supporting Information section at the end of the article. Fig S1. (A, B, C) Relapse stage flow results showed the blast cells were positive for HLA-DR, CD13, CD33, CD117 and CD34. (D) Empty vector and HA-tagged TFG-FGFR1 were expressed in 293T cells. Immunoblotting analysis of the expression of FGFR1, SHI1, BCL2 and KLF4 were performed in 293T cells. Signal quantification was performed using Ima-geJ software. For each antibody, we did multiple repeat experiments; only representative results are shown. (E) HL60 cell line with TFG-FGFR1 showed reduced cell viability when cultured with imatinib or ponatinib in vitro. The cellular viability was assessed after 48 h in culture with CCK-8. Nonlinear regression curves were fitted for evaluation of IC50 values. Table SI. Next-generation sequencing (a panel of 114 genes). Table SII. Mutations observed in the genes.References . (2013) Inhibition of TFG function causes hereditary axon degeneration by impairing endoplasmic reticulum structure.

Phase measuring is cumbersome and expensive, especially when operation frequency of array is high. To avoid phase measuring, a novel failed sensor localization algorithm using amplitude-only near-field data is proposed. In perspective of Bayesian theory, the maximum a posteriori estimation of array excitation leads to a mixed-norm minimization problem subject to a quadratic constraint. Iterative procedure based on alternating directions method of multipliers is used to solve this problem. Computer simulations show that the proposed algorithm is able to give accurate estimation of array excitation and precisely locate the failed sensors of the array using amplitude-only near-field data.

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Background: Patient-derived xenograft (PDX) models are biologically stable and can accurately reflect the primary tumor in histopathology and genetic expression in many cancers. In lung cancer, the models' engraftment rate by endobronchial ultrasound transbronchial needle aspiration (EBUS-TBNA) and computed tomography (CT)-guided biopsy is exceptionally low, and this limits the development of PDX models in lung cancer research. In this study, we aimed to improve the traditional method, and explore the feasibility and convenience of establishing lung cancer PDX models using the samples directly from surgical resection. We also endeavored to explore the correlation between PDX formation and primary tumor B7-H3 protein expression.Methods: From September 2017 to July 2018, 24 patients were enrolled in this study. The pathological diagnoses were as follows: 15 adenocarcinomas, 7 squamous cell carcinomas, 1 large cell carcinoma, and 1 small cell carcinoma. The tumor tissues were cut into sizes of 2×2×2 mm 3 /fragment and inoculated subcutaneously into immunodeficiency mice with a trocar needle. The engrafted tumors were passaged at least 3 generations, and B7-H3 IHC staining was performed on primary tumors. To explore the correlation between PDX formation and B7-H3 protein expression, we also performed H&E staining to evaluate whether the established PDX models could retain the histological features of the primary tumor.Results: Xenograft formation success was achieved in 19 out of 24 cases (79.2%). The time between implantation to tumor formation was an average of 81.5 days (27-154 days) in P1, an average of 44.4 days (14-122 days) in P2, and an average of 26.9 days (12-75 days) in P3. The diameter of the tumor was associated with tumor engraftment: the longer the diameter, the easier engraftment formation was (P=0.0342).The data also suggests that lung cancers with B7-H3 expression greatly induced PDX formation (P=0.0375).The histological characters of each passage resembled the primary tumors. Conclusions:This study shows that the samples from carefully selected lung cancer by surgical resection can be used for the establishment of PDX models with a high success rate; this improved method is closer to actual clinical work. Compared with the same kind of research, the success rate of the xenograft indicates significant improvement, so this improved method is suitable for promotion and application. The results of H&E staining showed that the histological characters of each passage resembled the primary tumors. Furthermore, the diameter of the tumor was associated with tumor engraftment, and higher B7-H3 expression demonstrated a statistically significant difference compared with the PDX model formation.

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