In this work we explore a new hydrogel stamp material obtained from polymerizing 2-hydroxyethyl acrylate and poly(ethylene glycol) diacrylate in the presence of water for the microcontact printing of proteins directly on gold substrates and by covalent coupling to self-assembled monolayers of alkanethiols. At high cross-link density, the hydrogel is rigid, hydrophilic, and with a high buffer holding capacity to enable the unsupported printing of protein patterns homogeneously and reproducibly, with micrometer-range precision. The stamps were used to print antibodies to human parathyroid hormone, which were shown using immunoassay tests to retain their biological function with binding capacities comparable to those of solution-adsorbed antibodies.
The optical properties of one-dimensional gradient-refractive-index lens arrays based on liquid crystals are studied. We find that it is quite possible, using theoretical methods, to predict angular distributions of the light emanating from such arrays when they are illuminated with collimated monochromatic light. We compare four theoretical methods in relation to experiments. The experimental data and the model, based on a combination of eikonal methods and diffraction, are in close correspondence. Features such as maximal beam width and number of extrema in the angular light distribution are discussed and explained theoretically. We also studied dispersion effects, both experimentally and theoretically, with good agreement between the two.
Integrating market knowledge in the architecture of product platforms can be a challenge for firms. In this study, we draw on knowledge‐based theory to propose the importance of bilateral knowledge: a marketer's knowledge of product platforms and a platform architect's knowledge of the market opportunity for securing marketing involvement and platform success. Along with these two personal coordination mechanisms, we examine the impact of a marketing department's level of product platform knowledge as a structural coordination mechanism and account for its moderating effects on these personal mechanisms. To test our model, we conducted a survey at a multinational electronics company, with the architects of product platforms as key informants. Our results show that an architect's knowledge of market opportunities for the product platform fuels marketing's involvement and improves financial performance, as does the marketer's knowledge of product platforms, although this is entirely mediated by marketing's involvement. A marketing department's product platform knowledge has a strong, direct and moderating impact; it acts as a substitute for the individual marketer's personal knowledge, yet enhances the impact of the architect's knowledge of the market opportunity. Therefore, innovation managers are advised to foster their marketing department's understanding of product platforms.
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