Considering the important role of oxidative stress in the pathogenesis of several neurological diseases, and the growing evidence of the presence of compounds with antioxidant properties in the plant extracts, the aim of the present study was to investigate the antioxidant capacity of three plants used in Brazil to treat neurological disorders: Melissa officinalis, Matricaria recutita and Cymbopogon citratus. The antioxidant effect of phenolic compounds commonly found in plant extracts, namely, quercetin, gallic acid, quercitrin and rutin was also examined for comparative purposes. Cerebral lipid peroxidation (assessed by TBARS) was induced by iron sulfate (10 microM), sodium nitroprusside (5 microM) or 3-nitropropionic acid (2 mM). Free radical scavenger properties and the chemical composition of plant extracts were assessed by 1'-1' Diphenyl-2' picrylhydrazyl (DPPH) method and by Thin Layer Chromatography (TLC), respectively. M. officinalis aqueous extract caused the highest decrease in TBARS production induced by all tested pro-oxidants. In the DPPH assay, M. officinalis presented also the best antioxidant effect, but, in this case, the antioxidant potencies were similar for the aqueous, methanolic and ethanolic extracts. Among the purified compounds, quercetin had the highest antioxidant activity followed by gallic acid, quercitrin and rutin. In this work, we have demonstrated that the plant extracts could protect against oxidative damage induced by various pro-oxidant agents that induce lipid peroxidation by different process. Thus, plant extracts could inhibit the generation of early chemical reactive species that subsequently initiate lipid peroxidation or, alternatively, they could block a common final pathway in the process of polyunsaturated fatty acids peroxidation. Our study indicates that M. officinalis could be considered an effective agent in the prevention of various neurological diseases associated with oxidative stress.
RESUMO:Fracionamento cromatográfi co da fração em diclorometano obtida do extrato etanólico das folhas de Vernonia tweediana Baker (Asteraceae) conduziu ao isolamento de α-amirina, β-amirina, lupeol, β-sitosterol, estigmasterol e espinasterol. As estruturas foram identifi cadas através de técnicas espectroscópicas usuais, além da comparação com dados relatados na literatura. Os compostos isolados são relatados pela primeira vez para a espécie V. tweediana. Unitermos:Vernonia tweediana, Asteraceae, triterpenos, esteróides.ABSTRACT: "Phytoconstituents isolated from dichloromethane fraction of Vernonia tweediana Baker leaves". Fractionation of the dichloromethane -soluble fraction from the ethanol extract of the leaves of Vernonia tweediana (Asteraceae) led to the isolation of α-amyrin, β-amyrin, lupeol, β-sitosterol, stigmasterol and spinasterol. The structures of the isolates were elucidated by spectroscopic analysis and comparison with literature data. The isolated compounds are reported for the fi rst time to the species V. tweediana.
Background: Lipid-Core Nanocapsules (LNC) containing co-encapsulated-coumaric acid and coumarin are under development. However, there is a lack of analytical methods to assay these bioactives in nanoformulations. Objective: The aim of this study was to validate an LC analytical method for the simultaneous determination of ρ-coumaric acid and coumarin in lipid-core nanocapsules. Methods: The mobile phase was composed of acetonitrile:water (40:60 v/v) adjusted to pH 4 and a C- 18 reversed-phase column was used. Both bioactives were detected at 275 nm. Specificity, linearity, range, precision and accuracy of the method were assessed, according to the official requirements. Results: Nanocapsules containing ρ-coumaric and coumarin had monomodal particle size distribution, spherical-shape and Z-average size of 207 ± 2 nm. LC method was specific, linear (5 to 30 µg.mL-1), precise (RSD < 5%) and accurate (97 - 103%). It was applied to assay the content and encapsulation efficiency of the bioactive substances in LNC, which were close to 0.5 mg.mL-1 and 72%, respectively. Conclusion: The proposed analytical method is reliable for the simultaneous assay of ρ-coumaric acid and coumarin in nanocapsules and can be further used in their development.
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