The surface-enhanced Raman scattering (SERS) spectra of polydrin of Trabala vishnou nuclear polyhedrosis virus (sample 1) and polydrin of Dendrolimus punctatus nuclear polyhedrosis virus (sample 2) adsorbed in silver hydrosol were investigated. The features in the adsorption mode, enhancement character and mechanism of the SERS spectra of these two samples were compared and are discussed. It was found that the molecules of sample 1 adsorbed on the silver surface through carboxyl and amino groups, and the molecules of sample 2 through the sulfur atom and also carboxyl and amino groups. The chemisorption of the molecules of both samples and their short-range character indicate that the molecular mechanism is the primary enhancement in the SERS spectra of molecules of these two insect viruses.
Raman spectra were obtained and analysed for polyhedrin and virion protein of Trabala vishnou nuclear polyhedrosis virus (TvNPV-P and TvNPV-VP, respectively). It was found that the secondary structures of the protein of the two samples differ: the protein of TvNPV-P has predomiuantly an a-helical and gsheet structure whereas that of TvNPV-P contains predominantly a gsheet content, with an a-helix content and a lower random-coil or fbturo content. All tyrosyl residues in the protein of TvNPV-P are exposed, whereas 80% of the tyrosyl residues are exposed and 20% are buried in the protein of TvNPV-VP. The majority of the tryptophan residues in the protein of TvNPV-VP are in a hydropbobic environment. The TvNPV-VP has a frans~uehe-fraizs configuration of the C-C-S-S-C-€ linkage.
The Surface-Enhanced Raman Scattering (SERS) spectra of the virions of four kinds of insect virus: Nuclear polyhedrosis virus of Trabala Vishnou (TvNPV), Nuclear polyhedrosis virus of Gynaephora ruoergensis (GrNPV), Granulosis Virus of preris rapae (PrGV) and Granulosis Virus of Darna trima (DtGV) adsorbed on the surface of silver colloid particles have been obtained and investigated. The strong interactions appeared between silver surface and C00 groups, 2 groups or aromatic side-chains in SERS of the molecules of the virions. The intensities of bands related to the vibrations of COO-groups are 458-.-460cn11: U ( COO ), 568-577cni': w( C00 ) , 6l4624cm': 6 ( COO ) 9l4934cnf' : U ( C-COO ) and the bands related to the vibrations of NH2 groups are 1 1 14-i 134cm': t(NH2), 1 62O-1632cm': 6 (NH2), 3390--3398cm1: u (NH2). It indicates that the molecules of the virions adsorbed on silver throughboth the COO-and 2 groups. The short-range enhancement results from a chemical interaction between the virion molecules and the silver surface. The bands at low frequency (2 1 8-236 cm) in the SERS spectra of the four samples show that chemiscorption is a main mechanism on silver hydrosis of the virion. The n -electron systems of side-chains of the aromatic amino acids in virion have formed the additional adsorption centers in the molecules of virions, it will decrease the distance between some groups ofmolecules and the silver surface. So the bands at 928 914 930 934 cm', due to the skeletal C-C vibration, have been enhanced.
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