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Objectives Phantom sound perception (tinnitus) may arise from altered brain activity within auditory cortex. Auditory cortex neurons in tinnitus animal models show increased spontaneous firing rates. This may be a core characteristic of tinnitus. Functional near‐infrared spectroscopy (fNIRS) has shown similar findings in human auditory cortex. Current fNIRS approaches with cap recordings are limited to ∼3 cm depth of signal penetration due to the skull thickness. To address this limitation, we present an innovative fNIRS approach via probes adapted to the external auditory canal. The adapted probes were placed deeper and closer to temporal lobe of the brain to bypass confining skull bone and improve neural recordings. Methods Twenty adults with tinnitus and 20 nontinnitus controls listened to periods of silence and broadband noise (BBN) during standard cap and adapted ear canal fNIRS neuroimaging. The evaluators were not blinded, but the protocol and postprocessing for the two groups were identical. Results Standard fNIRS measurements in participants with tinnitus revealed increased auditory cortex activity during silence that was suppressed during auditory stimulation with BBN. Conversely, controls displayed increased activation with noise but not during silence. Importantly, adapted ear canal fNIRs probes showed similar hemodynamic responses seen with cap probes in both tinnitus and controls. Conclusions In this proof of concept study, we have successfully fabricated, adapted, and utilized a novel fNIRS technology that replicates established findings from traditional cap fNIRS probes. This exciting new innovation, validated by replicating previous and current cap findings in auditory cortex, may have applications to future studies to investigate brain changes not only in tinnitus but in other pathologic states that may involve the temporal lobe and surrounding brain regions. Level of Evidence NA.

We present a novel, noninvasive, super-continuum infrared spectroscopy of cytochrome c oxidase (SCISCCO) system for simultaneously measuring hemodynamic and metabolic parameters, and we demonstrate its utility by applying it to lab calibration tests, human studies, and swine animal studies. The system optically assays the redox state of cytochrome c oxidase (CCO), as well as traditional markers including oxygenated (HbO) and deoxygenated (HbR) hemoglobin. To demonstrate in vivo feasibility, the measured responses of oxygenation and CCO responses to acute ischemia on the arm and forehead in human participants are compared to data from the literature. The validated SCISCCO system is then applied in human studies to measure cerebral oxygenation and the redox state of CCO in participants during an attention test protocol. We show that the redox state of CCO and hemodynamics measured by the SCISCCO system are consistent with the physiological hypothesis established in prior studies. To enable use of the SCISCCO system in laboratory and hospital settings as well as transportation to remote locations, a cart-based SCISCCO prototype system has also been developed. The cart-based SCISCCO prototype is applied to swine animal models undergoing induction of hemorrhagic shock followed by partial resuscitative endovascular balloon occlusion of the aorta (pREBOA). The pilot study demonstrates the feasibility of using the SCISCCO instrument within the context of existing protocols and validates the instrument’s measurements against the physiological and hemodynamic parameters measured by other conventional devices.

Objective Tinnitus, phantom sound perception, arises from aberrant brain activity within auditory cortex. In tinnitus animal models, auditory cortex neurons show increased spontaneous firing and neural synchrony. In humans, similar hyperactivation in auditory cortex has been displayed with functional near-infrared spectroscopy (fNIRS). Resting-state functional connectivity (RSFC) or increased connectivity between brain regions has also been shown in tinnitus using fNIRS. However, current fNIRS technology utilizes infrared (IR)-sources and IR-detectors placed on the scalp that restricts (~3 cm depth IR penetration) signal capture to outer cerebral cortex due to skin and skull bone. To overcome this limitation, in this proof of concept study, we adapted fNIRS probes to fit in the external auditory canal (EAC) to physically place IR-probes deeper within the skull thereby extracting neural signals from deeper auditory cortex. Methods Twenty adults with tinnitus and 20 nontinnitus controls listened to periods of silence and broadband noise before and after 5 min of silence to calculate RSFC. Concurrent scalp probes over auditory cortex and an adapted probe placed in the right EAC were utilized. Results For standard probes, left and right auditory cortex in tinnitus showed increased RSFC to each other and to other nonauditory cortices. Interestingly, adapted fNIRS probes showed trends toward increased RSFC. Conclusion While many areas for the adapted probes did not reach significance, these data using a highly innovative and newly created probe adapting fNIRS technology to the EAC substantiates our previously published data in human tinnitus and concurrently validates this technology as a useful and expanded brain imaging modality.

This study describes a contactless vital sign monitoring (CVSM) system capable of measuring heart rate (HR) and respiration rate (RR) using a low-power, indirect time-of-flight (ToF) camera. The system takes advantage of both the active infrared illumination as well as the additional depth information from the ToF camera to compensate for the motion-induced artifacts during the HR measurements. The depth information captures how the user is moving with respect to the camera and, therefore, can be used to differentiate where the intensity change in the raw signal is from the underlying heartbeat or motion. Moreover, from the depth information, the system can acquire respiration rate by directly measuring the motion of the chest wall during breathing. We also conducted a pilot human study using this system with 29 participants of different demographics such as age, gender, and skin color. Our study shows that with depth-based motion compensation, the success rate (system measurement within 10% of reference) of HR measurements increases to 75%, as compared to 35% when motion compensation is not used. The mean HR deviation from the reference also drops from 21 BPM to −6.25 BPM when we apply the depth-based motion compensation. In terms of the RR measurement, our system shows a mean deviation of 1.7 BPM from the reference measurement. The pilot human study shows the system performance is independent of skin color but weakly dependent on gender and age.