Localization microscopy vastly improves the resolution achieved by fluorescence microscopy by fitting the positions of individual fluorophores. We examine the reconstructions produced by different fitting algorithms for instances of fixed pattern noise--systematic tendencies to alter estimated emitter positions according to their subpixel location in a way that does not reflect the ground truth structure. We show that while not readily visible at standard empirical signal strengths, fixed pattern noise can occur when performing sub-pixel fitting, and that its degree varies according to the algorithm used and the relative size of the pixels compared to the point spread function. For pixel sizes in the range 80-170 nm, this results in variations in accuracy of the order of 2-4 nm-comparatively small for many applications, but non-negligible in scenarios where very high accuracy is sought.
The Short Primary Single-sided Linear Induction Motor (SLIM) applied in medium-low maglev vehicle has end effect that will cause a significant reduction of train thrust. This paper analyzes the relationship between the Longitudinal Dynamic End Effect (LDEE) and the speed of SLIM. It is concluded that LDEE increases with the motor speed. The LDEE at motor's exit area is called tailing effect. We take advantage of the tailing effect between adjacent SLIMs to make the air-gap magnetic field of these motors continuous by adjusting their intervals and excitation phase difference. The several SLIM on the same side of the vehicle is equivalent to a "whole motor" with overall one entry area and one exit area. This method can reduce the resistance of the whole vehicle running at high speed, which can provide a new solution for the design of high-speed SLIM applied in Maglev.
Keywords SLIM • LDEE • Eddy current field • Tailing effect • Maglev* Xuelong Liu
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