The
strong light–matter interactions between dyes and plasmonic
nanoantennas enable the study of fundamental molecular-optical processes.
Here, we overcome conventional limitations with high-throughput single-molecule
polarization-resolved microscopy to measure dye emission polarization
modifications upon near-field coupling to a gold nanorod. We determine
that the emission polarization distribution is not only rotated toward
the nanorod’s dominant localized surface plasmon mode as expected,
but it is also unintuitively broadened. With a reduced-order
analytical model, we elucidate how this distribution broadening depends
upon both far-field interference and off-resonant coupling between
the molecular dipole and the nanorod transverse plasmon mode. Experiments
and modeling reveal that a nearby plasmonic nanoantenna affects dye
emission polarization through a multicolor process, even when the
orthogonal plasmon modes are separated by approximately 3 times the
dye emission line width. Beyond advancing our understanding of plasmon-coupled
emission modifications, this work promises to improve high-sensitivity
single-molecule fluorescence imaging, biosensing, and spectral engineering.
AbstractBiomolecular condensates play a key role in organizing RNAs and proteins into membraneless organelles. Bacterial RNP-bodies (BR-bodies) are a biomolecular condensate containing the RNA degradosome mRNA decay machinery, but the biochemical function of such organization remains poorly defined. Here we define the RNA substrates of BR-bodies through enrichment of the bodies followed by RNA-seq. We find that long, poorly translated mRNAs, small RNAs, and antisense RNAs are the main substrates, while rRNA, tRNA, and other conserved ncRNAs are excluded from these bodies. BR-bodies stimulate the mRNA decay rate of enriched mRNAs, helping to reshape the cellular mRNA pool. We also observe that BR-body formation promotes complete mRNA decay, avoiding the build-up of toxic endo-cleaved mRNA decay intermediates. The combined selective permeability of BR-bodies for both, enzymes and substrates together with the stimulation of the sub-steps of mRNA decay provide an effective organization strategy for bacterial mRNA decay.
Spleen Tyrosine Kinase (SYK) is a critical immune signaling molecule and therapeutic target. We identified damaging monoallelic
SYK
variants in six patients with immune deficiency, systemic disease such as colitis, arthritis and skin inflammation, and diffuse large B cell lymphomas. The SYK variants increased phosphorylation and enhanced downstream signaling indicating gain-of-function. A knock-in (SYK
S544Y
) mouse model of a patient variant (p.S550Y) recapitulated aspects of the human disease that could be partially treated with a SYK inhibitor or transplantation of bone marrow from wildtype mice. Our studies demonstrate that SYK gain-of-function variants result in a potentially treatable form of inflammatory disease.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.