SARS-CoV-2, previously was named as COVID-2019 by the WHO, is now pandemic which has been reported 5,077 human death of 136,895 confirmed cases in 123 countries (updated on 14 March 2020 from WHO official website). The viruses have been successfully isolated, but the pathogenesis mechanisms and effective vaccines are undergoing extensively study. SARS-CoV-2 belongs to Betacoronavirus genera in the subfamily Orthocoronavirinae of family Coronaviridae, in which SARS-CoV and MERS-CoV are also in this group. The natural host of highly pathogenic SARS and MERS coronaviruses was confirmed as bats, and bats are also thought to be the natural hosts for SARS-CoV-2 based upon genomic sequence analysis (Wang, Horby, Hayden, & Gao, 2020). Coronaviruses needed intermediate hosts before being able to infect humans. Masked palm civets and dromedary camels were confirmed as intermediate hosts for SARS-CoV and MERS-CoV (Guarner, 2020), but the intermediate hosts remain unknown for SARS-CoV-2 (Ward, Li, & Tian, 2020). In order to find the intermediate host of SARS-CoV-2, a commercial double-antigen sandwich ELISA, which could be applied for different species of animals, was used to detect SARS-CoV-2-specific antibodies in different species of animals. Before applied to clinical serum samples, the sensitivity and specificity of kit were initially confirmed using SARS-CoV-2-positive and SARS-CoV-2-negative sera from experimental animals including rabbit, mouse, pig and ferret. SARS-CoV-2-negative sera from other species of experimental animals were also used which included chicken, duck, rat, guinea pig, beagle dog and rhesus monkey. After that, the kit was used to detect SARS-CoV-2-specific antibodies in domestic livestock (pig, cow, sheep, horse), poultry (chicken, duck, goose), experimental animals (mice, rat, guinea pig, rabbit and monkey), companion animal (dog and cat) and wild animals (camel, fox, mink, alpaca, ferret, bamboo rat, peacock, eagle, tiger rhinoceros, pangolin, leopard cat, jackal,