We investigated the diversity of euglossine bees in ten areas of Atlantic Forest Domain in São Paulo State, Brazil. Bees were collected with odor baits for 2 years, from March 2007 to March 2009. From a standardized effort during the first year of sampling, we compare the four areas using indexes of diversity, evenness, and similarity of euglossine communities. In the second year, we added six new places for presenting a general overview on the Atlantic forest in São Paulo. A total of 2,395 individuals of 23 species were recorded in the study areas. Ilhabela had the highest number of species (19) and individuals (1,260). The most attractive odor bait was eucalyptol, which attracted 16 species and 58 % of all individuals. Euglossa cordata (Linnaeus 1958) was the most abundant species, representing 50 % of the individuals sampled; apparently, populations of this species are more abundant at low altitudes, as for example on islands and on the coast. The results also suggest a difference in richness among altitudes, since coastal areas (where the climatic variation are milder) had more species. The present study advances knowledge on the occurrence of euglossine bees in the Atlantic Forest and fills the lack of a systematic study of these bees of coastal areas in São Paulo State.