Euglena sp. is a member of the Euglena genus that has been successfully isolated under extreme conditions. The pH level of Dieng Peatland is 2.0-3.5. that suitable for the growth of Euglena. There are several ways to increase biofuel production in Euglena sp. by increasing the production of lipids and fatty acids produced through cultivation engineering, metabolic engineering and genetic engineering. One of the efforts in increasing growth, biomass and lipid levels in microalgae, especially Euglena sp. is to provide nitrogen variation in his cultivation medium. The concentration of nitrogen applied to the culture medium is able to increase growth, biomass production and total lipids in microalgae. The aim of this article was to analyze the effect of nitrogen sources in form of Sodium Nitrate (NaNO3) on the growth of microalgae and biomass production. The results showed that NaNO3 stimulated Euglena sp. to produce the highest amount of biomass (0,377 gram/L) after adding 1,5 grams of NaNO3.
Euglena sp. is green microalgae in an acidic environment (pH 2.5-3.5). Euglena sp. has recently been developed widely in industry because of its capability to produce lipids that can be utilized to synthesize biofuel. Microalgae is a potential source of biodiesel, especially in the form of a consortium culture. One of the microalgae consortium cultures that have been explored is the nature consortium microalgae of Glagah strain. The Glagah consortia were isolated from Lagoon in the Glagah Beach, Kulonprogo, Yogyakarta. This study aimed to determine the total production of biomass, lipids, carbohydrates, and proteins of mixed culture of Glagah strain consortium and Euglena sp. as biodiesel substrate. The biomass test was measured using the dry weigh method using a filtration vacuum pump kit, lipids were measured using the Blight Dryer method by adding chloroform and methanol as solvents, carbohydrates were measured using the Sulfur Phenol Acid method by adding Phenol and sulfuric acid (H2SO4), and proteins were measured using the Bradford method by adding SDS and Bradford’s solution. The total production and productivity of biomass, lipids, carbohydrates, and proteins showed that the mixed culture of Glagah strain consortium with Euglena sp. was higher than the Glagah strain consortium. It reached 0.410 g/L; 0.253; 0.856 g/L; and 0.623 g/L. Therefore, it could be concluded that the mixed culture of the Glagah strain consortium with Euglena sp. could increase the production of biomass, lipids, carbohydrates, and protein up to two times that of the Glagah strain consortium so that this mixed culture treatment could be used as a reference in microalgae cultivation for biodiesel.
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