Aim: Many freshwater fishes are migrating poleward to more thermally suitable habitats in response to warming climates. In this study, we aimed to identify which freshwater fishes are most sensitive to climatic changes and asked: (i) how fast are lakes warming? (ii) how fast are fishes moving? and (iii) are freshwater fishes tracking climate?Location: Ontario, Canada.Methods: We assembled a database containing time series data on climate and species occurrence data from 10,732 lakes between 1986 and 2017. We calculated the rate of lake warming and climate velocity for these lakes. Climate velocities were compared with biotic velocities, specifically the rate at which the northernmost extent of each species shifted north. Results:Lakes in Ontario warmed by 0.2°C decade −1 on average, at a climate velocity of 9.4 km decade −1 between 1986 and 2017. In response, some freshwater fishes have shifted their northern range boundaries with considerable interspecific variation ranging from species moving southwards at a rate of −58.9 km decade −1 to species ranges moving northwards at a rate of 83.6 km decade −1 over the same time period.More freshwater fish species are moving into northern lakes in Ontario than those being lost. Generally, predators are moving their range edges northwards, whereas prey fishes are being lost from northern lakes. Main Conclusions:The concurrent loss of cooler refugia, combined with antagonistic competitive and predatory interactions with the range expanding species, has resulted in many commercially important predators moving their range edges northwards, whereas prey species have contracted their northern range edge boundaries.Trophic partitioning of range shifts highlights a previously undocumented observation of the loss of freshwater fishes from lower trophic levels in response to climate-driven migrations.