Thomas Trichtinger scite author profile

The substrate specificity of 2'-deoxy-2'-substituted uridines and their 5'-phosphates towards thymidylate synthetase from Escherichia coli K12 was investigated. Besides the natural substrate 2'-deoxyuridine-5'-phosphate (dUMP), only 2'-deoxy-2'-fluorouridine-5'-phosphate (dUf IMP) was a substrate. The KM of dUfIMP is 11 times higher than that of dUMP, while the V values are virtually the same. It is concluded that the size of the 2'-su9gituent and not its polarity (and the concomitant conformational change) determines substrate specificity of thymidylate synthetase.

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The p-Nitrophenylethyl Group - An Universal Blocking Group in Nucleoside and Nucleotide Chemistry

Pfleiderer

Himmelsbach

Charubala

et al. 1985

Nucleosides and Nucleotides

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Thomas Trichtinger

The p-nitrophenylethyl (NPE) group

Synthesis of O6-p-nitrophenylethyl guanosine and 2′-deoxyguanosine derivatives

ChemInform Abstract: THE P‐NITROPHENYLETHYL (NPE) GROUP. A VERSATILE NEW BLOCKING GROUP FOR PHOSPHATE AND AGLYCONE PROTECTION IN NUCLEOSIDES AND NUCLEOTIDES

2′-Deoxy-2′-fluorouridine-5′-phosphate: an alternative substrate for thymidylate synthetase from Escherichia coli K12

The p-Nitrophenylethyl Group - An Universal Blocking Group in Nucleoside and Nucleotide Chemistry

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