Antibody-dependent lymphocyte cytotoxicity (ADLC) was demonstrated against Epstein-Barr virus (EBV)-infected RAJI cells with peripheral with peripheral blood lymphocytes from EBV-infected donors. No cytotoxic activity was detected against unifected RAJl cells. The results indicated that this antibody-mediated cytotoxic reaction was directed against the same EBV-induced membrane antigens (MA) previously defined by the membrane immunofluorescence (MF) assay. Antibody to EBV-associated early antigens did not participate in this in vitro reaction. Antibody titers to EBV-induced MA were significantly higher by the ADLC assay in comparison with the MF test. A preliminary study showed no relationship between high antibody titers and the presence of EBV-associated malignancies. The possible in vivo significance of this immune reaction was discussed.

major groups of Herpesvirus saimiri (HVS)associated antigens [early antigens (EA), late antigens (LA), membrane antigens (MA)] in 10 owl monkeys infected with HVS were related to the clinical course of HVS-induced disease. Results are also presented which show that EA is produced 4-8 hours earlier than LA in HVSinfected cells providing further evidence that these were 2 distinct groups of antigens. In animals that developed neoplasms, antibodies against all 3 groups of antigens were found;

The association between the induction of antibodies to early antigens (EA) with the detection of virusinfected peripheral blood lymphocytes, and the possible correlation of these factors with the response of lymphocytes to different mitogens was investigated in owl monkeys infected with Herpesvirus saimiri (HVS). There was a positive correlation between the production of antibodies to EA and the quantitative recovery of virus from peripheral blood lymphocytes. The loss of responsiveness of lymphocytes from leukemic owl monkeys to T-cell mitogens paralleled these two parameters suggesting that virus genome-carrying lymphocytes are functionally altered.

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